LOCUS       sequence-120840-         847 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TALEN expression construct for targeting the murine ROSA26 locus. It
            recognize the sequence: GGGAGTCTTCTGGGC. Can be used with the pBS SK
            mCherryROSAbsr plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 847)
  TITLE     rosa26 knock-in plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCAGGS-ROSAr
FEATURES             Location/Qualifiers
     source          1..847
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     27..46
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      75..84
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             96..116
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             126..149
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             150..176
                     /codon_start=1
                     /product="epitope tag from the baculovirus Autographa 
                     californica GP64 envelope fusion protein"
                     /label=AcV5 tag
                     /translation="SWKDASGWS"
ORIGIN
        1 cttcttcttt ttcctacagc tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt
       61 tggcaaagaa ttccgccacc atggcttcct cccctccaaa gaaaaagaga aaggttgcgg
      121 ccgctgacta caaggatgac gacgataaaa gttggaagga cgcaagtggt tggtctagaa
      181 tgcatgcggc cccgcgacgg cgtgctgcgc aaccctccga cgcttcgccg gccgcgcagg
      241 tggatctacg cacgctcggc tacagtcagc agcagcaaga gaagatcaaa ccgaaggtgc
      301 gttcgacagt ggcgcagcac cacgaggcac tggtgggcca tgggtttaca cacgcgcaca
      361 tcgttgcgct cagccaacac ccggcagcgt tagggaccgt cgctgtcacg tatcagcaca
      421 taatcacggc gttgccagag gcgacacacg aagacatcgt tggcgtcggc aaacagtggt
      481 ccggcgcacg cgccctggag gccttgctca cggatgcggg ggagttgaga ggtccgccgt
      541 tacagttgga cacaggccaa cttgtgaaga ttgcaaaacg tggcggcgtg accgcaatgg
      601 aggcagtgca tgcatcgcgc aatgcactga cgggtgcccc cctgaacctg accccggacc
      661 aagtggtggc tatcgccagc aacaatggcg gcaagcaagc gctcgaaacg gtgcagcggc
      721 tgttgccggt gctgtgccag gaccatggcc tgactccgga ccaagtggtg gctatcgcca
      781 gccacgatgg cggcaagcaa gcgctcgaaa cggtgcagcg gctgttgccg gtgctgtgcc
      841 aggacca
//
