LOCUS       sequence-120841-         833 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MBII85 snoRNA expression cassett, contains snoRNA in natural intron 
            surrounded by natural exons. To improve snoRNA expression efficiency
            5' splice site was mutated to consensus sequence..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Falaleeva M, Surface J, Shen M, de la Grange P, Stamm S
  TITLE     SNORD116 and SNORD115 change expression of multiple genes and modify
            each other's activity.
  JOURNAL   Gene. 2015 Jul 26. pii: S0378-1119(15)00848-3. doi: 
            10.1016/j.gene.2015.07.023.
  PUBMED    26220404
REFERENCE   2  (bases 1 to 833)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene. 2015 
            Jul 26. pii: S0378-1119(15)00848-3. doi: 
            10.1016/j.gene.2015.07.023."
COMMENT     Sequence Label: pcDNA3.1-MBII85-5'SS mut
FEATURES             Location/Qualifiers
     source          1..833
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     35..54
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        35..53
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ctagagaacc cactgcttac tggcttatcg aaattaatac gactcactat agggagaccc
       61 aagctggcta gcgtttaaac ttaagcttgg taccggttgc attccctttc cagtatggtc
      121 catggaaaac atgcagagga aatggcccca cagcattggc tgagtgttta tgtcgtccaa
      181 ctgagtctcc atcgaactgt tgtggtgttg ctgacttgcc ctaggtcacc tgagttaaaa
      241 ggtaagttat atgggaattg ctgcatgaat tctaggtgcc caggtcttgg tggagcaggg
      301 aacagctgcc attgattatg tccaggccct tcgggacatg gagcatgcct agggcctctg
      361 gtttctccca tgttttgagc tttggcccat tgttccctgg cagtggattg tcagtgtcca
      421 gcagtccatt gctctcccca aagccagagc actccagagt actggaacca cttaatgcaa
      481 tgcactccaa cgagcttgga tctatgatga ttcccagtca aacattcctt ggaaaagctg
      541 aacaaaatga gtgaaaactc tgtaccgcca ctctcatcgg aactgaggtc cagcatcttg
      601 ggtctcccag gtgctatggg cgtcaggcac aggctgccca aacactccat tgttcttgac
      661 ttcttcgggc tctagccaga ctctccagag acaggtgtgt gcttggccta ctctgccaaa
      721 aattcatggt gtaggacaca gcaggtcctt aagcactggt ggcttgctcc ctttgctgta
      781 gtggagatgc ttggcagata ctcagtaggt agtcatgcta ggtccaagga gag
//
