LOCUS       sequence-120842-         841 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MBII85 snoRNA expression cassett, contains snoRNA in natural intron 
            sorounded by natural exons. To improve snoRNA expression efficiency 
            3' splice site was mutated to consensus sequences..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 841)
  AUTHORS   Falaleeva M, Surface J, Shen M, de la Grange P, Stamm S
  TITLE     SNORD116 and SNORD115 change expression of multiple genes and modify
            each other's activity.
  JOURNAL   Gene. 2015 Jul 26. pii: S0378-1119(15)00848-3. doi: 
            10.1016/j.gene.2015.07.023.
  PUBMED    26220404
REFERENCE   2  (bases 1 to 841)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene. 2015 
            Jul 26. pii: S0378-1119(15)00848-3. doi: 
            10.1016/j.gene.2015.07.023."
COMMENT     Sequence Label: pcDNA3.1(+)MBII-85-3'ss mut
FEATURES             Location/Qualifiers
     source          1..841
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     32..51
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        32..50
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gagaacccac tgcttactgg cttatcgaaa ttaatacgac tcactatagg gagacccaag
       61 ctggctagcg tttaaactta agcttggtac cggttgcatt ccctttccag tatggtccat
      121 ggaaaacatg cagaggaaat ggccccacag cattggctga gtgtttatgt cgtccaactg
      181 agtctccatc gaactgttgt ggtgttgctg acttgcccta ggtcacctga gttaaatggt
      241 aaggcacatg ggaattgctg catgaattct aggtgcccag gtcttggtgg agcagggaac
      301 agctgccatt gattatgtcc aggcccttcg ggacatggag catgcctagg gcctctggtt
      361 tctcccatgt tttgagcttt ggcccattgt tccctggcag tggattgtca gtgtccagca
      421 gtccattgct ctccccaaag ccagagcact ccagagtact ggaaccactt aatgcaatgc
      481 actccaacga gcttggatct atgatgattc ccagtcaaac attccttgga aaagctgaac
      541 aaaatgagtg aaaactctgt accgccactc tcatcggaac tgaggtccag catcttgggt
      601 ctcccaggtg ctatgggcgt caggcacagg ctgcccaaac actccattgt tcttgacttc
      661 ttcgggctct agccagactc tccagagaca ggtgtgtgct tggcctactc tgccaaaaat
      721 tcatggtgta ggacacagca ggtccttaag cactggtggc ttgctccctt tgctgtagtg
      781 gagatgcttg gcagatactc agtaggtagt catgctaggt ccaaggagag ggccccagcc
      841 a
//
