LOCUS       sequence-120843-         807 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  antisense RNA probe for RNase protection or nothern.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 807)
  AUTHORS   Falaleeva M, Surface J, Shen M, de la Grange P, Stamm S
  TITLE     SNORD116 and SNORD115 change expression of multiple genes and modify
            each other's activity.
  JOURNAL   Gene. 2015 Jul 26. pii: S0378-1119(15)00848-3. doi: 
            10.1016/j.gene.2015.07.023.
  PUBMED    26220404
REFERENCE   2  (bases 1 to 807)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene. 2015 
            Jul 26. pii: S0378-1119(15)00848-3. doi: 
            10.1016/j.gene.2015.07.023."
COMMENT     Sequence Label: pCRII-MBII85 probe
FEATURES             Location/Qualifiers
     source          1..807
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(249..267)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(250..267)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(285..301)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(285..301)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(298..320)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    309..325
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(333..339,340..357,358..363))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    378..399
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(516..533)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(767..786)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agggcgaatt gggccctcta gatgcatgct cgagcggccg ccagtgtgat ggatatctgc
       61 agaattcgcc cttgctggac ctcagttccg atgagagtgg cggtacagag ttttcactca
      121 ttttgttcag cttttccaag gaatgtttga ctgggaatca tcatagatca agggcgaatt
      181 ccagcacact ggcggccgtt actagtggat ccgagctcgg taccaagctt gatgcatagc
      241 ttgagtattc tatagtgtca cctaaatagc ttggcgtaat catggtcata gctgtttcct
      301 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
      361 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
      421 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
      481 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
      541 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
      601 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
      661 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
      721 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
      781 tttccccctg gaagctccct cgtgcgc
//
