LOCUS       sequence-120846-         852 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  antisense RNA probe for RNase protection or nothern.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 852)
  AUTHORS   Falaleeva M, Surface J, Shen M, de la Grange P, Stamm S
  TITLE     SNORD116 and SNORD115 change expression of multiple genes and modify
            each other's activity.
  JOURNAL   Gene. 2015 Jul 26. pii: S0378-1119(15)00848-3. doi: 
            10.1016/j.gene.2015.07.023.
  PUBMED    26220404
REFERENCE   2  (bases 1 to 852)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene. 2015 
            Jul 26. pii: S0378-1119(15)00848-3. doi: 
            10.1016/j.gene.2015.07.023."
COMMENT     Sequence Label: pCR4-MBII52-probe
FEATURES             Location/Qualifiers
     source          1..852
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(164..182)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(203..219)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(203..219)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(216..238)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    227..243
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(251..257,258..275,276..281))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    296..317
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(434..451)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(685..704)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agggcgaatt gaatttagcg gccgcgaatt cgccctttcc cgggcctcag cgtaatccta
       61 ttgagcatga attttatgtc atcacctttc ttcatgacat tgggttgtca tcattgaccc
      121 agcccgggaa agggcgaatt cgtttaaacc tgcaggacta gtccctttag tgagggttaa
      181 ttctgagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
      241 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
      301 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
      361 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
      421 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
      481 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
      541 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
      601 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
      661 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
      721 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
      781 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
      841 gctccaagct gg
//
