LOCUS       sequence-120847-         770 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  antisense RNA probe for RNase protection or nothern.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 770)
  AUTHORS   Falaleeva M, Surface J, Shen M, de la Grange P, Stamm S
  TITLE     SNORD116 and SNORD115 change expression of multiple genes and modify
            each other's activity.
  JOURNAL   Gene. 2015 Jul 26. pii: S0378-1119(15)00848-3. doi: 
            10.1016/j.gene.2015.07.023.
  PUBMED    26220404
REFERENCE   2  (bases 1 to 770)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene. 2015 
            Jul 26. pii: S0378-1119(15)00848-3. doi: 
            10.1016/j.gene.2015.07.023."
COMMENT     Sequence Label: pCR4-MBII52-probe
FEATURES             Location/Qualifiers
     source          1..770
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(175..194)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(176..194)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(201..218)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(201..217)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(210..232)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             355..657
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="QFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKV
                     SRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     536..555
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 accctcacta aagggactag tcctgcaggg tttaaacgaa ttcgcccttt cccgggctgg
       61 gtcaatgatg acaacccaat gtcatgaaga aaggtgatga cataaaattc atgctcaata
      121 ggattacgct gaggcccggg aaagggcgaa ttcgcggccg ctaaattcaa ttcgccctat
      181 agtgagtcgt attacaattc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
      241 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
      301 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctatacgt acggcagttt
      361 aaggtttaca cctataaaag agagagccgt tatcgtctgt ttgtggatgt acagagtgat
      421 attattgaca cgccggggcg acggatggtg atccccctgg ccagtgcacg tctgctgtca
      481 gataaagtct cccgtgaact ttacccggtg gtgcatatcg gggatgaaag ctggcgcatg
      541 atgaccaccg atatggccag tgtgccggtc tccgttatcg gggaagaagt ggctgatctc
      601 agccaccgcg aaaatgacat caaaaacgcc attaacctga tgttctgggg aatataaatg
      661 tcaggcatga gattatcaaa aaggatcttc acctagatcc ttttcacgta gaaagccagt
      721 ccgcagaaac ggtgctgacc ccggatgaat gtcagctact gggctatctg
//
