LOCUS       sequence-120848-         775 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MBII85 snoRNA expression cassett, contains snoRNA in natural intron 
            sorounded by natural exons. To improve snoRNA expression efficiency 
            3' splice site was mutated to consensus sequences..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Falaleeva M, Surface J, Shen M, de la Grange P, Stamm S
  TITLE     SNORD116 and SNORD115 change expression of multiple genes and modify
            each other's activity.
  JOURNAL   Gene. 2015 Jul 26. pii: S0378-1119(15)00848-3. doi: 
            10.1016/j.gene.2015.07.023.
  PUBMED    26220404
REFERENCE   2  (bases 1 to 775)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene. 2015 
            Jul 26. pii: S0378-1119(15)00848-3. doi: 
            10.1016/j.gene.2015.07.023."
COMMENT     Sequence Label: pcDNA3.1(+)MBII-85-3'ss mut
FEATURES             Location/Qualifiers
     source          1..775
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 actcaggtga cctagggcaa gtcagcaaca ccacaacagt tcgatggaga ctcagttgga
       61 cgacataaac tctcagccaa tgctgtgggg ccatttcctc tgcatgtttt ctatggatca
      121 tactggaaag ggaatgcaac cctgtgaaga gaaaagagag aacatgcccc gattacagtg
      181 ggaccaatgc aaaaccacat ggattatggg cttaccatgt actatgggct tgtcatatct
      241 cacaggcacc ctcaacactc ctgctcttac caacctcttc acactgcccc tccagatctc
      301 acttgctgtt actctcaggc cttggatggg aacatgccat gccctttgct ctcaagtgct
      361 tgctgggtcc tcagctcact ctaaggactt aaggatgata tgtgagctgc aatacaggta
      421 ctcaacactt tctcttaggt ccatgccatc aacttcttcc accagtgtcc tgtgcaaagt
      481 tttcaaggga atgaaggctc ttctccaagc atccagtcag aaaaagccaa taacaagggc
      541 aatcataggt ccccttgcct atgctgacaa agaaggagga ggcagctctg aataactcca
      601 ccagcaaaat tgggcctcca aggatatcta agccaacaac ttttgagcct tcaatcatgg
      661 ctccagtcct tctctgatca ttacttctgt gtctttgcat gaggtaaaat caagctcctc
      721 tgagagttca gaactccagg tcatgggaag caggcttcat tttcacagct gaaag
//
