LOCUS       sequence-120850-         725 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MBII85 snoRNA expression cassett, contains snoRNA in natural intron 
            sorounded by natural exons. To improve snoRNA expression efficiency 
            5' and 3' splice sites were mutated to consensus sequences..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 725)
  AUTHORS   Falaleeva M, Surface J, Shen M, de la Grange P, Stamm S
  TITLE     SNORD116 and SNORD115 change expression of multiple genes and modify
            each other's activity.
  JOURNAL   Gene. 2015 Jul 26. pii: S0378-1119(15)00848-3. doi: 
            10.1016/j.gene.2015.07.023.
  PUBMED    26220404
REFERENCE   2  (bases 1 to 725)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene. 2015 
            Jul 26. pii: S0378-1119(15)00848-3. doi: 
            10.1016/j.gene.2015.07.023."
COMMENT     Sequence Label: pcDNA3.1(+)MBII-85-5'/3'ss mut
FEATURES             Location/Qualifiers
     source          1..725
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gtgacctagg gcaagtcagc aacaccacaa cagttcgatg gagactcagt tggacgacat
       61 aaactctcag ccaatgctgt ggggccattt cctctgcatg ttttctatgg atcatactgg
      121 aaagggaatg caaccctgtg aagagaaaag agagaacatg ccccgattac agtgggacca
      181 atgcaaaacc acatggatta tgggcttacc atgtactatg ggcttgtcat atctcacagg
      241 caccctcaac actcctgctc ttaccaacct cttcacactg cccctccaga tctcacttgc
      301 tgttactctc aggccttgga tgggaacatg ccatgccctt tgctctcaag tgcttgctgg
      361 gtcctcagct cactctaagg acttaaggat gatatgtgag ctgcaataca ggtactcaac
      421 actttctctt aggtccatgc catcaacttc ttccaccagt gtcctgtgca aagttttcaa
      481 gggaatgaag gctcttctcc aagcatccag tcagaaaaag ccaataacaa gggcaatcat
      541 aggtcccctt gcctatgctg acaaagaagg aggaggcagc tctgaataac tccaccagca
      601 aaattgggcc tccaaggata tctaagccaa caacttttga gccttcaatc atggctccag
      661 tccttctctg atcattactt ctgtgtcttt gcatgaggta aaatcaagct cctctgagag
      721 ttcag
//
