LOCUS       sequence-120852-        1086 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A pCS2 based vector to generate a 3'-3HA tag using GATEWAY cloning 
            technology.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1086)
  AUTHORS   Grant IM, Balcha D, Hao T, Shen Y, Trivedi P, Patrushev I, Fortriede
            JD, Karpinka JB, Liu L, Zorn AM, Stukenberg PT, Hill DE, Gilchrist 
            MJ
  TITLE     The Xenopus ORFeome: A resource that enables functional genomics.
  JOURNAL   Dev Biol. 2015 Dec 15;408(2):345-57. doi: 
            10.1016/j.ydbio.2015.09.004. Epub 2015 Sep 29.
  PUBMED    26391338
REFERENCE   2  (bases 1 to 1086)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ydbio.2015.09.004"; journalName: "Dev Biol"; date: 
            "2015-12-15- 15"; volume: "408"; issue: "2"; pages: "345-57"
COMMENT     Sequence Label: pCSF107mT-GATEWAY-3'-3HA
FEATURES             Location/Qualifiers
     source          1..1086
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(132..256)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     regulatory      264..273
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             276..365
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     primer_bind     complement(400..419)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(401..419)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    424..558
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(448..467)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     502..521
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(709..729)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(709..727)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(748..764)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(748..764)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(761..783)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    772..788
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(796..802,803..820,821..826))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    841..862
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(979..996)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ggtctccgtt atcggggaag aagtggctga tctcagccac cgcgaaaatg acatcaaaaa
       61 cgccattaac ctgatgttct ggggaatata aatgtcaggc tcccttatac acagccagtc
      121 tgcaggtcga ccatagtgac tggatatgtt gtgttttaca gtattatgta gtctgttttt
      181 tatgcaaaat ctaatttaat atattgatat ttatatcatt ttacgtttct cgttcagctt
      241 tcttgtacaa agtggttggt accgccacca tggcttaccc atacgatgtt cctgactatg
      301 cgggctatcc ctatgacgtc ccggactatg caggatccta tccatatgac gttccagatt
      361 acgctgaatt ctagctcgag tctagaatcg agcctctcgc cctatagtga gtcgtattac
      421 gtagatccag acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag
      481 tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata
      541 agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg
      601 gaggtgtggg aggtttttta attcgcggcg cgccgcggtt ccatctgttt tcttatctgt
      661 tctttcatct gttcttttat ctgtttgttt ggtacccagc ttttgttccc tttagtgagg
      721 gttaattgcg cgcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
      781 gctcacaatt ccacacaaca tacgagccgg gagcataaag tgtaaagcct ggggtgccta
      841 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
      901 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
      961 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     1021 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     1081 aggaaa
//
