LOCUS       sequence-120861-         561 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 561)
  AUTHORS   Stanton BC, Nielsen AA, Tamsir A, Clancy K, Peterson T, Voigt CA
  TITLE     Genomic mining of prokaryotic repressors for orthogonal logic gates.
  JOURNAL   Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
  PUBMED    24316737
REFERENCE   2  (bases 1 to 561)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411."
COMMENT     Sequence Label: pET21a-DhaR
FEATURES             Location/Qualifiers
     source          1..561
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             34..51
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             61..84
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
ORIGIN
        1 actttaagaa ggagatatac catgggcagc agccatcatc atcatcatca cagcagcggc
       61 ctggaagttc tgttccaggg gccccatatg gcacgtacac cggttcgtca gcatctggtt
      121 gaaaaaggca cccaggtttt tctggaacgt ggttatagcg gtagcgcagt tcaggatatt
      181 accgcagcag cagaagttcc gaaaggtagc ttttataacc actttgaaag caaagaagcc
      241 tttggcggtc aggttctgca agaatttttc agcgatctgc agcgtaccat tagcagcacc
      301 ctggaagatg caagcgttcc gcctgttcgt cgtctgcaga actattttgc agcaattatt
      361 gaaaccctgg atggtcaggg ttgtctgatt ggtaatttta gcgttgaact gagtccgctg
      421 agtgatgttg ttcgtaccga actgctgcgt atttttgaac agtgggttaa accgttccag
      481 aaatgcatta ttgaaggtca agaaaccggt agcattcgtc gtgatctggc accggatctg
      541 ctggcagatt ttctgattgc a
//
