LOCUS       sequence-120863-         425 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 425)
  AUTHORS   Stanton BC, Nielsen AA, Tamsir A, Clancy K, Peterson T, Voigt CA
  TITLE     Genomic mining of prokaryotic repressors for orthogonal logic gates.
  JOURNAL   Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
  PUBMED    24316737
REFERENCE   2  (bases 1 to 425)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411."
COMMENT     Sequence Label: pET21a-AmrR
FEATURES             Location/Qualifiers
     source          1..425
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             34..51
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             61..84
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
ORIGIN
        1 actttaagaa ggagatatac catgggcagc agccatcatc atcatcatca cagcagcggc
       61 ctggaagttc tgttccaggg gccccatatg gcacgtaaaa ccaaagaaga aagccagaaa
      121 acccgtgatg gtattctgga tgcagcagaa cgtgtttttc tggaaaaagg tgttggcacc
      181 accgcaatgg cagatctggc agatgcagcc ggtgttagcc gtggtgcagt ttatggtcac
      241 tacaaaaaca aaatcgaagt ttgtctggcc atgtgtgatc gtgcatttgg tcagattgaa
      301 gttccggatg aaaatgcacg tgttccggca ctggatattc tgctgcgtgc aggtatgggt
      361 tttctgcgtc agtgttgtga accgggtagc gttcagcgtg ttctggaaat tctgtatctg
      421 aaatg
//
