LOCUS       sequence-120864-        1143 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A pCS2 based vector to generate a 3'-LAP (eGFP-TEV-S) tag using 
            GATEWAY cloning technology.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1143)
  AUTHORS   Grant IM, Balcha D, Hao T, Shen Y, Trivedi P, Patrushev I, Fortriede
            JD, Karpinka JB, Liu L, Zorn AM, Stukenberg PT, Hill DE, Gilchrist 
            MJ
  TITLE     The Xenopus ORFeome: A resource that enables functional genomics.
  JOURNAL   Dev Biol. 2015 Dec 15;408(2):345-57. doi: 
            10.1016/j.ydbio.2015.09.004. Epub 2015 Sep 29.
  PUBMED    26391338
REFERENCE   2  (bases 1 to 1143)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ydbio.2015.09.004"; journalName: "Dev Biol"; date: 
            "2015-12-15- 15"; volume: "408"; issue: "2"; pages: "345-57"
COMMENT     Sequence Label: pCSF107mT-GATEWAY-3'-LAP tag
FEATURES             Location/Qualifiers
     source          1..1143
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(132..256)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     regulatory      285..294
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(291..293,294..296,297..1007)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(291..293,294..296,297..1007)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(336..357)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(597..616)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     944..965
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             1053..1073
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1086..1130
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
ORIGIN
        1 ggtctccgtt atcggggaag aagtggctga tctcagccac cgcgaaaatg acatcaaaaa
       61 cgccattaac ctgatgttct ggggaatata aatgtcaggc tcccttatac acagccagtc
      121 tgcaggtcga ccatagtgac tggatatgtt gtgttttaca gtattatgta gtctgttttt
      181 tatgcaaaat ctaatttaat atattgatat ttatatcatt ttacgtttct cgttcagctt
      241 tcttgtacaa agtggttggt accgagctcg gatcgctacc ggtcgccacc atggtgagca
      301 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
      361 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga
      421 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
      481 ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact
      541 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg
      601 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
      661 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
      721 acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg
      781 tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc
      841 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca
      901 cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
      961 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtcc ggaggggatc
     1021 ggtggagctc caccggtggc ggccgctcta gagagaatct ttattttcag ggcgccgcca
     1081 aattcaaaga aaccgctgct gctaaattcg aacgccagca catggacagc ggaggtggat
     1141 aac
//
