LOCUS       sequence-120865-         833 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a cysteine-free version of pET30Xa-SBL1(160E) (4Cys to 
            Ser) with N-term Histag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Coffa G, Imber AN, Maguire BC, Laxmikanthan G, Schneider C, Gaffney 
            BJ, Brash AR
  TITLE     On the relationships of substrate orientation, hydrogen abstraction,
            and product stereochemistry in single and double dioxygenations by 
            soybean lipoxygenase-1 and its Ala542Gly mutant.
  JOURNAL   J Biol Chem. 2005 Nov 18;280(46):38756-66. Epub 2005 Sep 12.
  PUBMED    16157595
REFERENCE   2  (bases 1 to 833)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2005-11-18"; volume: "280(46)"; pages: "38756-66. 
            Epub 2005 Sep 12"
COMMENT     Sequence Label: pET30Xa-SBL1(160E,NoCys)
FEATURES             Location/Qualifiers
     source          1..833
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(16..33)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     regulatory      644..653
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 agcagccgga tctcagtggt ggtggtggtg gtgctcgagt gcggccgcaa gcttgtcgac
       61 ggagctcgaa ttcggatccg atatcgccat ggccagagga gagttagagc cccatgacta
      121 gtttagatag agatgctatt tggaattccc ctaaaagtta acccttcctc actgctagga
      181 taaagcaaag tgtatggcag ttgaaccggg ccaagtcgat tgccctgcag actcggatca
      241 ttgttcctcc tcacaagttt ttcctcaatt tccttgagct tgtttccaaa cttttgaaag
      301 gcttgtaatg cttttgaatc agaggtccaa tgtgggttgt ccctctggcc aaggtagacc
      361 tcatcagaag catgtgtcga caagatctct atcactgaaa ggctaatgag agtcggcaac
      421 tttgatgtaa ttgttctcaa ataagccttt tcatggttat taatcatttc ttcatattct
      481 ggggtgcctt tctcaggaag caaccttcta gaagcagttg ggcggttcat tatcaaacct
      541 ccataaggat actgaccaaa attaacggct gcatggagag ctgaagcaat ccatatgata
      601 atgagggaaa cttcaacaag gtcttcaagt gtctgcaact taggccacca tggcttgtct
      661 ttcaaatcac catggccttt ctctacagct tctttccacc aatgttggag ttcagaatca
      721 ttcttgacat catcatctct tgcatagtac aagggcacat attcttgaac ccatgtctta
      781 attgcagccc atatctccag tccatcagcg gcataaggat agtcctctat cag
//
