LOCUS       sequence-120875-         966 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Standard for cell-free expression benchmarking..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 966)
  AUTHORS   Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y,
            Alexandrov K
  TITLE     Performance benchmarking of four cell-free protein expression 
            systems.
  JOURNAL   Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814.
  PUBMED    26301602
REFERENCE   2  (bases 1 to 966)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814."
COMMENT     Sequence Label: pCellFree_G03 TCF25
FEATURES             Location/Qualifiers
     source          1..966
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(18..41)
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     primer_bind     complement(184..203)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(185..203)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        235..339
                     /gene="bla"
                     /label=AmpR promoter
     CDS             524..902
                     /label=ISS
                     /note="immunostimulatory sequence from the AmpR gene; 
                     contains unmethylated CpG dinucleotides in the context of 
                     5'-AACGTT-3' (Sato et al., 1996)"
     primer_bind     complement(558..577)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 tcgcccttgc ttgtgccgtg gtgatggtgg tgatggtggt gaccagaggt gcccatggtt
       61 tcacttacgt gtttaatgtc tttacagact ttatacatta ctgtcatggt taaataaaat
      121 aaaataaaat aaaataaaat aaaataaaat aaaataaaat aaaataaaat aaacttaaga
      181 tgtccctata gtgagtcgta ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
      241 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
      301 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
      361 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
      421 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
      481 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
      541 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
      601 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
      661 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
      721 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
      781 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
      841 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
      901 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcacaa taaatagact
      961 ggatgg
//
