LOCUS       sequence-120881-         832 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Tucker KL, Beard C, Dausmann J, Jackson-Grusby L, Laird PW, Lei H, 
            Li E, Jaenisch R
  TITLE     Germ-line passage is required for establishment of methylation and 
            expression patterns of imprinted but not of nonimprinted genes.
  JOURNAL   Genes Dev. 1996 Apr 15. 10(8):1008-20.
  PUBMED    8608936
REFERENCE   2  (bases 1 to 832)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            1996 Apr 15. 10(8):1008-20."
COMMENT     Sequence Label: pPGKpuro
FEATURES             Location/Qualifiers
     source          1..832
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        4..22
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     4..21
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(679..699)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
ORIGIN
        1 gctatttagg tgacactata gaatactcaa gcttatgcat gcggccgcat ctagagtcga
       61 ccagcttctg atggaattag aacttggcaa aacaatactg agaatgaagt gtatgtggaa
      121 cagaggctgc tgatctcgtt cttcaggcta tgaaactgac acatttggaa accacagtac
      181 ttagaaccac aaagtgggaa tcaagagaaa aacaatgatc ccacgagaga tctatagatc
      241 tatagatcat gagtgggagg aatgagctgg cccttaattt ggttttgctt gtttaaatta
      301 tgatatccaa ctatgaaaca ttatcataaa gcaatagtaa agagccttca gtaaagagca
      361 ggcatttatc taatcccacc ccacccccac ccccgtagct ccaatccttc cattcaaaat
      421 gtaggtactc tgttctcacc cttcttaaca aagtatgaca ggaaaaactt ccattttagt
      481 ggacatcttt attgtttaat agatcatcaa tttctgcatc ccggggatct gatatcatcg
      541 atgcatgggg tcgtgcgctc ctttcggtcg ggcgctgcgg gtcgtggggc gggcgtcagg
      601 caccgggctt gcgggtcatg caccaggtgc gcggtccttc gggcacctcg acgtcggcgg
      661 tgacggtgaa gccgagccgc tcgtagaagg ggaggttgcg gggcgcggag gtctccagga
      721 aggcgggcac cccggcgcgc tcggccgcct ccactccggg gagcacgacg gcgctgccca
      781 gacccttgcc ctggtggtcg ggcgagacgc cgacggtggc caggaaccac gc
//
