LOCUS       sequence-120886-         944 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Standard for cell-free expression benchmarking..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 944)
  AUTHORS   Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y,
            Alexandrov K
  TITLE     Performance benchmarking of four cell-free protein expression 
            systems.
  JOURNAL   Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814.
  PUBMED    26301602
REFERENCE   2  (bases 1 to 944)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814."
COMMENT     Sequence Label: pCellFree_G03 STAT3
FEATURES             Location/Qualifiers
     source          1..944
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    168..192
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ggtaccacgc gtacccaaca cacggacaca tgcacgaaca cgcatgcaga gagatcatac
       61 atcatctata gagaagtaca cgtaaaagta agactcgtcg tctgccagct acaaccgaga
      121 aggcgacgtg aaaggaacaa gaaaggagga ggagggcggc cgcttacacc actttgtaca
      181 agaaagttgg gcacatgggg gaggtagcgc actccgaggt caactccatg tcaaaggtga
      241 gggactcaaa ctgccctcct gctgagggtt cagcaccttc accattattt ccaaactgca
      301 tcaatgaatc taaagtgcgg ggggacatcg gcaggtcaat ggtattgctg caggtcgttg
      361 gtgtcacaca gataaacttg gtcttcaggt atggggcagc gcctgggtca gcttcaggat
      421 gctcctggct ctctggccga caatactttc cgaatgcctc ctccttggga atgtcaggat
      481 agagatagac cagtggagac accaggatat tggtagcatc catgatctta tagcccatga
      541 tgatttcagc aaatgacatg ttgttcagct gctgctttgt gtatggttcc acggactgga
      601 tctgggtctt accgctgatg tccttctcca cccaagtgaa agtgacgcct ccttctttgc
      661 tgctttcact gaatcttagc aggaaggtgc ctggaggctt agtgctcaag atggcccgct
      721 cccgctcctt actgataaag cccatgatgt acccttcgtt ccaaagggcc aggatgtact
      781 ttttcacaag gtcaatgata ttgtccagcc agacccagaa ggagaagccc ttgccagcca
      841 tgttttcttt gcaaaattta gcccatgtga tctgacaccc tgaataattc acaccaggtc
      901 ccaagagttt ctctgccagt gtagtcagct gctcgatgct cagt
//
