LOCUS       sequence-120889-         890 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Standard for cell-free expression benchmarking..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y,
            Alexandrov K
  TITLE     Performance benchmarking of four cell-free protein expression 
            systems.
  JOURNAL   Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814.
  PUBMED    26301602
REFERENCE   2  (bases 1 to 890)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814."
COMMENT     Sequence Label: pCellFree_G03 AKT1
FEATURES             Location/Qualifiers
     source          1..890
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    168..192
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ggtaccacgc gtacccaaca cacggacaca tgcacgaaca cgcatgcaga gagatcatac
       61 atcatctata gagaagtaca cgtaaaagta agactcgtcg tctgccagct acaaccgaga
      121 aggcgacgtg aaaggaacaa gaaaggagga ggagggcggc cgcttacacc actttgtaca
      181 agaaagttgg gcaggccgtg ccgctggccg agtaggagaa ctgggggaag tggggcctgc
      241 gctcgctgtc cacacactcc atgctgtcat cttggtcagg tggtgtgatg gtgatcatct
      301 gggccgtgaa ctcctcatca aaatacctgg tgtcagtctc cgacgtgacc tggggcttga
      361 agggtgggct gagcttcttc tcgtacacgt gctgccacac gataccggca aagaagcgat
      421 gctgcatgat ctccttggcg tcctcggagc ccccgccaag cctctgcttg gggtccttct
      481 tgagcagccc tgaaagcaag gacttggcct cgggaccaag cgtgcgcggg aagcggatct
      541 cctccatgag gatgagctca aaaagcttct catggtcctg gttgtagaag ggcaggcgac
      601 cgcacatcat ctcgtacatg accacgccca gcccccacca gtccactgca cggccgtagt
      661 cattgtcctc cagcacctcg ggggccaggt actcaggtgt gccgcaaaag gtcttcatgg
      721 tggcaccgtc cttgatcccc tccttgcaca gcccgaagtc tgtgatctta atgtgcccgt
      781 ccttgtccag catgaggttc tccagcttga ggtcccggta caccacgttc ttctccgagt
      841 gcaggtagtc cagggctgac acaatctcag cgccatagaa gcgggcccgg
//
