LOCUS       sequence-120890-         869 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Standard for cell-free expression benchmarking..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 869)
  AUTHORS   Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y,
            Alexandrov K
  TITLE     Performance benchmarking of four cell-free protein expression 
            systems.
  JOURNAL   Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814.
  PUBMED    26301602
REFERENCE   2  (bases 1 to 869)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814."
COMMENT     Sequence Label: pCellFree_G03 CYP2A13
FEATURES             Location/Qualifiers
     source          1..869
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    167..191
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ggtccacgcg tacccaacac acggacacat gcacgaacac gcatgcagag agatcataca
       61 tcatctatag agaagtacac gtaaaagtaa gactcgtcgt ctgccagcta caaccgagaa
      121 ggcgacgtga aaggaacaag aaaggaggag gagggcggcc gcttacacca ctttgtacaa
      181 gaaagctggg cccatgaggc ctgagcgggg caggaagctc atggtgtagt ttcgtgggat
      241 cgtggcaaag cccacgtgtt tgggggacac gtcgatatcc ttaggcgact gaggggactt
      301 gaagcgaaag ttctgcatga tggtggtgaa gaagagaaag agctccattc tggccaggcc
      361 ttctccaaaa cagtaccgct ttccgatgga aaagggcaca aaagcatcac tcttcttaaa
      421 ctgccccttc ttatccagga agtgctgggg attgaagtcc cgggggttgg agaagaacct
      481 ggggtctctc agcacggagc ccagcatagg gaacacttca gtgcccttag ggaggaagaa
      541 atcccgaaac ttggtgtcct tgttgaccct gtgggccaaa cccatgggga gcatgtctcc
      601 aaatctttgg atctcgtgga tcactgcctc tgtgtagggc atcttggccc ggtcctcaaa
      661 cttgggctgc cggttcttgc cgatcactct gtcaatctcc tcatggacct tggcctccac
      721 ctctgggtgc ttcatgagca gcaggaaacc gtagcgcagg gtggtgctca cggtctcagt
      781 gcccgcaaag aagaggttca gggtggtcat caccaggttc ttcaagtaga actctgtgtt
      841 ggggttcttc tcctcctcct gcatgcgga
//
