LOCUS       sequence-120891-         945 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Standard for cell-free expression benchmarking..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 945)
  AUTHORS   Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y,
            Alexandrov K
  TITLE     Performance benchmarking of four cell-free protein expression 
            systems.
  JOURNAL   Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814.
  PUBMED    26301602
REFERENCE   2  (bases 1 to 945)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814."
COMMENT     Sequence Label: pCellFree_G03 BMPR1A
FEATURES             Location/Qualifiers
     source          1..945
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    168..192
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ggtaccacgc gtacccaaca cacggacaca tgcacgaaca cgcatgcaga gagatcatac
       61 atcatctata gagaagtaca cgtaaaagta agactcgtcg tctgccagct acaaccgaga
      121 aggcgacgtg aaaggaacaa gaaaggagga ggagggcggc cgcttacacc actttgtaca
      181 agaaagttgg gcagattttt acatcttggg attcaaccat cttggcaagc gtcttcttaa
      241 ttctcaatgc tgtgagtctg gaggctggat tgtgggccca gcattctgac attagcttca
      301 aaactgctcg tagacattca tcactgttcc accgattaga cacaattggc cgcaaacgtt
      361 tgacacacac aacctcacgc atatcttcgt atgacggatc actcggtacc atgttgtaat
      421 atggcaattg gtattcttcc acgatccctc ctgtgataca acgacgagcc atctcccaaa
      481 tgattaggcc gaagctgtag atgtcagcca tgatgtaggg ctggaagtgg tttttgttca
      541 ggctttcgtc cagcacttcg ggagccatgt agcgtttggt gcccaccctg gtattcaagg
      601 gcacatcaac ttcatttgtg tcactgttga atttaacagc aaggcccagg tcagcaatgc
      661 agcaactccc atttttcttg atgaggatgt ttttgctctt taggtctcga tgagcaattg
      721 cgggctttcc ttgggtgcca taaatttctg tgtgcaggtg gcacagacca caggcagctg
      781 aataagccaa tttaagcagg gctctggtgt ccagtgtagc acatttcagg aagtcataga
      841 gagatccatt ttcatggtaa tcagtaatca aatagagctg agtccaggaa cctgtacctt
      901 taatgtctgc cgctatgaaa ccaagtatgt tttcatggcg catta
//
