LOCUS       sequence-120893-         900 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Standard for cell-free expression benchmarking..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y,
            Alexandrov K
  TITLE     Performance benchmarking of four cell-free protein expression 
            systems.
  JOURNAL   Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814.
  PUBMED    26301602
REFERENCE   2  (bases 1 to 900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814."
COMMENT     Sequence Label: pCellFree_G03 CYP3A4
FEATURES             Location/Qualifiers
     source          1..900
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    168..192
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ggtaccacgc gtacccaaca cacggacaca tgcacgaaca cgcatgcaga gagatcatac
       61 atcatctata gagaagtaca cgtaaaagta agactcgtcg tctgccagct acaaccgaga
      121 aggcgacgtg aaaggaacaa gaaaggagga ggagggcggc cgcttacacc actttgtaca
      181 agaaagttgg gcaggctcca cttacggtgc catcccttga ctcaaccttt agaacaacgg
      241 gtttttctgg ttgaagaagt cctcctaagc ttaatttcag ggggatctgt gtttctttac
      301 aaggtttgaa ggagaagttc tgaaggactc tgattagagc aagtttcatg ttcatgagag
      361 caaacctcat gccaatgcag tttctgggtc cacttccaaa gggtgtgtat atgtaaggat
      421 ctatgttgtc cttgttcttc ttgctgaatc tttcagggag gaacttctca ggctctgtcc
      481 agtactttgg gtcacggtga agagcatagc ttggaatcat caccaccacc cctttgggaa
      541 tgaacatccc attgatctca acatcttttt tgcagaccct ctcaagtctc atagcaattg
      601 ggaataatct gagcgtttca ttcaccacca tgtcaagata ctccatctgt agcacagtat
      661 cataggtggg tggtgcctta ttgggtaaaa ctgcatcaat ttcctcctgc agtttctgct
      721 ggacatcagg gtgagtggcc agttcataca taatgaagga gagaacactg ctcgtggttt
      781 catagccagc aaaaataaag ataattgatt gggccacgag ctccagatcg gacagagctt
      841 tgtgggactc agtttctttt gaattctgag agtcaatcat cagctgaagg aaatccactc
//
