LOCUS       sequence-120897-         820 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Standard for cell-free expression benchmarking..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y,
            Alexandrov K
  TITLE     Performance benchmarking of four cell-free protein expression 
            systems.
  JOURNAL   Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814.
  PUBMED    26301602
REFERENCE   2  (bases 1 to 820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814."
COMMENT     Sequence Label: pCellFree_G03 TCF12
FEATURES             Location/Qualifiers
     source          1..820
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    168..192
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ggtaccacgc gtacccaaca cacggacaca tgcacgaaca cgcatgcaga gagatcatac
       61 atcatctata gagaagtaca cgtaaaagta agactcgtcg tctgccagct acaaccgaga
      121 aggcgacgtg aaaggaacaa gaaaggagga ggagggcggc cgcttacacc actttgtaca
      181 agaaagttgg gtacatatga cccatagggt tggtagtttc actaagccca ggatgggttc
      241 ctggcagtgt ggttggcggc tctgccgata cggcagaaac tttttcttct tcccttctct
      301 taaggcaggc tgctttgggg ttaaggttcc tctctctgac ttgctgttct agactaagga
      361 tgactgccac ggcttgatga agaataagga gttttgtttg gggtttttca ctcttcaagt
      421 gaagctgaca cattcggcca agctctttga atgcttcatt aatatcccgc acgcgtaagc
      481 gttctctggc attgttagcc atccgcctct ccttctccct ttctatcttc tgttcagggt
      541 tcaaatcctc atcttcatta gtactgcttg ttctgcctct agatgaaacc ttgatatctt
      601 tttgggagga ttcatcatct gacttcatgt catctgatga aggaggttca tgaaggtttt
      661 catccttttc tttgttttca gtcttgattt ctgttgtaac aacagttcca gactgacttt
      721 gcaagccacc tctataattt tcttgtgttt tatggttcag gtctgtgctt gaagtagtga
      781 ctgtactaga caggactgaa tgattgccat tgagactgac
//
