LOCUS       sequence-120898-         891 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Standard for cell-free expression benchmarking..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 891)
  AUTHORS   Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y,
            Alexandrov K
  TITLE     Performance benchmarking of four cell-free protein expression 
            systems.
  JOURNAL   Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814.
  PUBMED    26301602
REFERENCE   2  (bases 1 to 891)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814."
COMMENT     Sequence Label: pCellFree_G03 TCF25
FEATURES             Location/Qualifiers
     source          1..891
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    168..192
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ggtaccacgc gtacccaaca cacggacaca tgcacgaaca cgcatgcaga gagatcatac
       61 atcatctata gagaagtaca cgtaaaagta agactcgtcg tctgccagct acaaccgaga
      121 aggcgacgtg aaaggaacaa gaaaggagga ggagggcggc cgcttacacc actttgtaca
      181 agaaagttgg gcagtcccac tccccctccc cctcagcgtc gtcctcgtgc ggtgcctcca
      241 ggtcgttgag gtggaagttg gccatcatgt cgcgcacagc cagcatcagc ctgttcaggc
      301 cctggttgcg gttcagaccc ccagccactc cttcctcggg cctctccccc tccatggtat
      361 agtttggcaa cagtgaccgg aagaagagag caatggtgtt tccatggctg ataggactta
      421 gcctctctgg cctgacgtag gagtagattg tgtccgaagg aggcagagga tcaaacccca
      481 tcacagactg cgtggtcacg tccgggggca gggcagcgac ggcttccttg atctcagaga
      541 ggatcacatg gcggtggata ttcctgggtg cacgctggta gagcaccttc cgccggttct
      601 cacaggcttc cacggctggg tccccggcgt ccactgcttg cagaacctcg tggacgttct
      661 cctccagcca gctcatggtg gcgggctctt tccagagaaa gtgtgacctc ccaaggtaca
      721 ggttcaccag ctggctcagg gcagggggct ggcttatttc agcattgggt ccaaagaagc
      781 ggtgactgga aacgctggcg tcgggccgca cactgcaaga ctcgagcagg ggcaggagga
      841 ctccagggaa catggtgagc gcctgctgta tcaggagaga ggccttctgc c
//
