LOCUS       sequence-120899-         897 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Standard for cell-free expression benchmarking..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 897)
  AUTHORS   Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y,
            Alexandrov K
  TITLE     Performance benchmarking of four cell-free protein expression 
            systems.
  JOURNAL   Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814.
  PUBMED    26301602
REFERENCE   2  (bases 1 to 897)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814."
COMMENT     Sequence Label: pCellFree_G03 PRKCA
FEATURES             Location/Qualifiers
     source          1..897
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    168..192
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ggtaccacgc gtacccaaca cacggacaca tgcacgaaca cgcatgcaga gagatcatac
       61 atcatctata gagaagtaca cgtaaaagta agactcgtcg tctgccagct acaaccgaga
      121 aggcgacgtg aaaggaacaa gaaaggagga ggagggcggc cgcttacacc actttgtaca
      181 agaaagttgg caatactgca ctctgtaaga tggggtgcac aaactggggg ttgacatacg
      241 agaacccttc aaaatcagac tggtctatgt tagcaataac cagctgatca ggtggtgtta
      301 agacgggctg tcctcgtgtg aagaacttgt caaagttctc tgctcctttg ccacacactt
      361 tgggcttgaa tggtggctgg atctccctgt tctccagttt ttcccagtcg atcctccgga
      421 agaaggcatg ctctctcacg tccctctccc cctcaggccc acagcccagc cgcttggctg
      481 ggtgtttggt catcagtcct ttgcagatag aaacagcctc cttggacaag gattttggat
      541 aggaaacgtt gtgctccatg atagactgaa atagctcgtc ttcatcttca ccatcaaatg
      601 gaggctgccc ggcaagcatt tcatacaaca ggacgccata ggcccaccag tccacagatt
      661 ttccatacgg ctgataagcg attatctctg gggcgatata atctggagtc ccacagaagg
      721 tcctggtcgt gactccatcc atcatgtgtt ccttgcacat cccaaagtca gcaattttga
      781 tatgtccttc tgaatccaac atgacgttat ctaacttcag atccctataa atgattcctc
      841 ttttatgaag aaagaacaat ccgatggaaa tctctgccgc atagaatact gcttgtg
//
