LOCUS       sequence-120904-         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Inducible expression of a mutant of PIAL2M where the SIM interacting
            motif 1 (VFDL 425-428) was substituted with alanines..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Tomanov K, Zeschmann A, Hermkes R, Eifler K, Ziba I, Grieco M, 
            Novatchkova M, Hofmann K, Hesse H, Bachmair A
  TITLE     Arabidopsis PIAL1 and 2 promote SUMO chain formation as E4-type SUMO
            ligases and are involved in stress responses and sulfur metabolism.
  JOURNAL   Plant Cell. 2014 Nov;26(11):4547-60. doi: 10.1105/tpc.114.131300. 
            Epub 2014 Nov 18.
  PUBMED    25415977
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1105/tpc.114.131300"; journalName: "Plant Cell"; date: 
            "2014-11"; volume: "26"; issue: "11"; pages: "4547-60"
COMMENT     Sequence Label: pMAL-PIAL2M-sim1
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             18..29
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(722..739)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(722..738)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(731..753)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 ataacaacaa cctcgggatc gagggaagga tttcagaatt cggatccgaa aaaccggttc
       61 ttaaagatta ccttcagtcc ggagtcattg aagcaagtcc agattcagac atcattgagg
      121 ggccatcacg agtatctctc agttgcccta taagtcgcaa gcggatcaag ttaccagtca
      181 agggccagtt atgtaaacat cttcagtgtt ttgatttctc aaactacgtt cacataaata
      241 tgaggaaccc aacctggcgc tgcccgcatt gcaatcaacc tgtttgctac cctgacattc
      301 gtttagatca aaacatggcc aagatattaa aagatgtgga acacaatgct gctgatgtaa
      361 tcatcgatgc tggtggtaca tggaaggtta caaaaaatac tggcgagacc ccggaacctg
      421 tgcgtgagat cattcatgat ctagaagacc cgatgagctt attaaactct ggtcctgttg
      481 ctgcagctgc tacgggggat gatgatgctg aactggaagt ttttggtgac aacaaggttg
      541 aggaccggaa gccctgtatg tctgatgctc agggtcaatc taataataac aacacaaata
      601 aacatccttc aaacgatgat tactcttcga tatttgatat ctctgatgtg atcgcacttg
      661 acccggagat tttatctgct ttgggaaaca ctgcgtgagt cgacctgcag gcaagcttgg
      721 cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc
      781 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
//
