LOCUS       sequence-120905-         764 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Inducible expression of a mutant of PIAL2M where the SIM interacting
            motif 1 (VFDL 425-428) was substituted with alanines..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 764)
  AUTHORS   Tomanov K, Zeschmann A, Hermkes R, Eifler K, Ziba I, Grieco M, 
            Novatchkova M, Hofmann K, Hesse H, Bachmair A
  TITLE     Arabidopsis PIAL1 and 2 promote SUMO chain formation as E4-type SUMO
            ligases and are involved in stress responses and sulfur metabolism.
  JOURNAL   Plant Cell. 2014 Nov;26(11):4547-60. doi: 10.1105/tpc.114.131300. 
            Epub 2014 Nov 18.
  PUBMED    25415977
REFERENCE   2  (bases 1 to 764)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1105/tpc.114.131300"; journalName: "Plant Cell"; date: 
            "2014-11"; volume: "26"; issue: "11"; pages: "4547-60"
COMMENT     Sequence Label: pMAL-PIAL2M-sim1
FEATURES             Location/Qualifiers
     source          1..764
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(644..655)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(707..730)
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
ORIGIN
        1 aaatctccgg gtcaagtgcg atcacatcag agatatcaaa tatcgaagag taatcatcgt
       61 ttgaaggatg tttatttgtg ttgttattat tagattgacc ctgagcatca gacatacagg
      121 gcttccggtc ctcaaccttg ttgtcaccaa aaacttccag ttcagcatca tcatcccccg
      181 tagcagctgc agcaacagga ccagagttta ataagctcat cgggtcttct agatcatgaa
      241 tgatctcacg cacaggttcc ggggtctcgc cagtattttt tgtaaccttc catgtaccac
      301 cagcatcgat gattacatca gcagcattgt gttccacatc ttttaatatc ttggccatgt
      361 tttgatctaa acgaatgtca gggtagcaaa caggttgatt gcaatgcggg cagcgccagg
      421 ttgggttcct catatttatg tgaacgtagt ttgagaaatc aaaacactga agatgtttac
      481 ataactggcc cttgactggt aacttgatcc gcttgcgact tatagggcaa ctgagagata
      541 ctcgtgatgg cccctcaatg atgtctgaat ctggacttgc ttcaatgact ccggactgaa
      601 ggtaatcttt aagaaccggt ttttcggatc cgaattctga aatccttccc tcgatcccga
      661 ggttgttgtt attgttattg ttgttgttgt tcgagctcga attagtctgc gcgtctttca
      721 gggcttcatc gacagtctga cgaccgctgg cggcgttgat cacc
//
