LOCUS       sequence-120907-         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Inducible expression of PIAL2 in E.coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Tomanov K, Zeschmann A, Hermkes R, Eifler K, Ziba I, Grieco M, 
            Novatchkova M, Hofmann K, Hesse H, Bachmair A
  TITLE     Arabidopsis PIAL1 and 2 promote SUMO chain formation as E4-type SUMO
            ligases and are involved in stress responses and sulfur metabolism.
  JOURNAL   Plant Cell. 2014 Nov;26(11):4547-60. doi: 10.1105/tpc.114.131300. 
            Epub 2014 Nov 18.
  PUBMED    25415977
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1105/tpc.114.131300"; journalName: "Plant Cell"; date: 
            "2014-11"; volume: "26"; issue: "11"; pages: "4547-60"
COMMENT     Sequence Label: pMAL-PIAL2M-wt
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             20..31
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(724..741)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(724..740)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(733..755)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 acataacaac aacctcggga tcgagggaag gatttcagaa ttcggatccg aaaaaccggt
       61 tcttaaagat taccttcagt ccggagtcat tgaagcaagt ccagattcag acatcattga
      121 ggggccatca cgagtatctc tcagttgccc tataagtcgc aagcggatca agttaccagt
      181 caagggccag ttatgtaaac atcttcagtg ttttgatttc tcaaactacg ttcacataaa
      241 tatgaggaac ccaacctggc gctgcccgca ttgcaatcaa cctgtttgct accctgacat
      301 tcgtttagat caaaacatgg ccaagatatt aaaagatgtg gaacacaatg ctgctgatgt
      361 aatcatcgat gctggtggta catggaaggt tacaaaaaat actggcgaga ccccggaacc
      421 tgtgcgtgag atcattcatg atctagaaga cccgatgagc ttattaaact ctggtcctgt
      481 tgttttcgat cttacggggg atgatgatgc tgaactggaa gtttttggtg acaacaaggt
      541 tgaggaccgg aagccctgta tgtctgatgc tcagggtcaa tctaataata acaacacaaa
      601 taaacatcct tcaaacgatg attactcttc gatatttgat atctctgatg tgatcgcact
      661 tgacccggag attttatctg ctttgggaaa cactgcgtga gtcgacctgc aggcaagctt
      721 ggcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa
      781 tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg
//
