LOCUS       sequence-120908-         831 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a cysteine-free version of WTsoybean lipoxygenase-1 160S 
            (4Cys to Ser) with N-term Histag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Coffa G, Imber AN, Maguire BC, Laxmikanthan G, Schneider C, Gaffney 
            BJ, Brash AR
  TITLE     On the relationships of substrate orientation, hydrogen abstraction,
            and product stereochemistry in single and double dioxygenations by 
            soybean lipoxygenase-1 and its Ala542Gly mutant.
  JOURNAL   J Biol Chem. 2005 Nov 18;280(46):38756-66. Epub 2005 Sep 12.
  PUBMED    16157595
REFERENCE   2  (bases 1 to 831)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2005-11-18"; volume: "280(46)"; pages: "38756-66. 
            Epub 2005 Sep 12"
COMMENT     Sequence Label: pET30Xa-SBL1(160S,NoCys)
FEATURES             Location/Qualifiers
     source          1..831
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(15..32)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     regulatory      643..652
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gcagccggat ctcagtggtg gtggtggtgg tgctcgagtg cggccgcaag cttgtcgacg
       61 gagctcgaat tcggatccga tatcgccatg gccagaggag agttagagcc ccatgactag
      121 tttagataga gatgctattt ggaattcccc taaaagttaa cccttcctca ctgctaggat
      181 aaagcaaagt gtatggcagt tgaaccgggc caagtcgatt gccctgcaga ctcggatcat
      241 tgttcctcct cacaagtttt tcctcaattt ccttgagctt gtttccaaac ttttgaaagg
      301 cttgtaatgc ttttgaatca gaggtccaat gtgggttgtc cctctggcca aggtagacct
      361 catcagaagc atgtgtcgac aagatctcta tcactgaaag gctaatgaga gtcggcaact
      421 ttgatgtaat tgttctcaaa taagcctttt catggttatt aatcatttct tcatattctg
      481 gggtgccttt ctcaggaagc aaccttctag aagcagttgg gcggttcatt atcaaacctc
      541 cataaggata ctgaccaaaa ttaacggctg catggagagc tgaagcaatc catatgataa
      601 tgagggaaac ttcaacaagg tcttcaagtg tctgcaactt aggccaccat ggcttgtctt
      661 tcaaatcacc atggcctttc tctacagctt ctttccacca atgttggagt tcagaatcat
      721 tcttgacatc atcatctctt gcatagtaca agggcacata ttcttgaacc catgtcttaa
      781 ttgcagccca tatctccagt ccatcagcgg cataaggata gtcctctatc a
//
