LOCUS       sequence-120916-         773 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  URA3 vector for cloning guide sequences using BclI-SwaI sites into 
            guide RNA expression cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 773)
  AUTHORS   Laughery MF, Hunter T, Brown A, Hoopes J, Ostbye T, Shumaker T, 
            Wyrick JJ
  TITLE     New vectors for simple and streamlined CRISPR-Cas9 genome editing in
            Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2015 Dec;32(12):711-20. doi: 10.1002/yea.3098. Epub 2015 Sep 
            21.
  PUBMED    26305040
REFERENCE   2  (bases 1 to 773)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/yea.3098"; 
            journalName: "Yeast"; date: "2015-12"; volume: "32"; issue: "12"; 
            pages: "711-20"
COMMENT     Sequence Label: pT040
FEATURES             Location/Qualifiers
     source          1..773
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     49..65
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     50..66
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(112..128)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(112..128)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     terminator      complement(203..222)
                     /label=SUP4 terminator
                     /note="transcription terminator for the S. cerevisiae SUP4 
                     tRNA gene"
     promoter        complement(304..572)
                     /label=SNR52 promoter
                     /note="promoter for the S. cerevisiae small nucleolar RNA 
                     gene SNR52"
     primer_bind     complement(598..617)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(599..617)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(624..641)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(624..640)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(633..655)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 ttaaccctca ctaaagggaa caaaagctgg taccgggccc cccctcgatc gaggtcgacg
       61 gtatcgataa gcttgatatc gaattcctgc agcccccgct cgagcggggg ggatccacta
      121 gttctagagc ggccgtggta tcgtttagat tggcaattac agtgtcttag ctcacatgct
      181 tataactaat tacatgactc gaagacataa aaaacaaaaa aagcaccacc gactcggtgc
      241 cactttttca agttgataac ggactagcct tattttaact tgctatttaa atgcatcgat
      301 tctgatcatt tatctttcac tgcggagaag tttcgaacgc cgaaacatgc gcaccaactt
      361 tcacttctac agcgtttgac caaaatcttt tgaacagaac attgtagggt gtgaaaaaat
      421 gcgcaccttt accgctagcc caagagggca ctacaaaatc tagagttgta cttcaaacgt
      481 acatgtaatc accttgtata tactcgaaag aaaacatcaa gtttctgtat aaatatgagt
      541 gaaagcataa tcatacatta tcttttcaaa gattgtccgc ggtggagctc caattcgccc
      601 tatagtgagt cgtattacaa ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac
      661 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
      721 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tgg
//
