LOCUS       sequence-120918-         775 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  High copy Cas9 expression vector with LEU2 marker for yeast 
            transformation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Laughery MF, Hunter T, Brown A, Hoopes J, Ostbye T, Shumaker T, 
            Wyrick JJ
  TITLE     New vectors for simple and streamlined CRISPR-Cas9 genome editing in
            Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2015 Dec;32(12):711-20. doi: 10.1002/yea.3098. Epub 2015 Sep 
            21.
  PUBMED    26305040
REFERENCE   2  (bases 1 to 775)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/yea.3098"; 
            journalName: "Yeast"; date: "2015-12"; volume: "32"; issue: "12"; 
            pages: "711-20"
COMMENT     Sequence Label: pJH001
FEATURES             Location/Qualifiers
     source          1..775
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      109..118
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tctactttta tagttagtct tttttttagt tttaaaacac caagaactta gtttcgaata
       61 aacacacata aacaaacaaa agatctgata tggatcgaat tagatctcgc caccatggac
      121 aagaagtatt ctatcggact ggacatcggg actaatagcg tcgggtgggc cgtgatcact
      181 gacgagtaca aggtgccctc taagaagttc aaggtgctcg ggaacaccga ccggcattcc
      241 atcaagaaaa atctgatcgg agctctcctc tttgattcag gggagaccgc tgaagcaacc
      301 cgcctcaagc ggactgctag acggcggtac accaggagga agaaccggat ttgttacctt
      361 caagagatat tctccaacga aatggcaaag gtcgacgaca gcttcttcca taggctggaa
      421 gaatcattcc tcgtggaaga ggataagaag catgaacggc atcccatctt cggtaatatc
      481 gtcgacgagg tggcctatca cgagaaatac ccaaccatct accatcttcg caaaaagctg
      541 gtggactcaa ccgacaaggc agacctccgg cttatctacc tggccctggc ccacatgatc
      601 aagttcagag gccacttcct gatcgagggc gacctcaatc ctgacaatag cgatgtggat
      661 aaactgttca tccagctggt gcagacttac aaccagctct ttgaagagaa ccccatcaat
      721 gcaagcggag tcgatgccaa ggccattctg tcagcccggc tgtcaaagag ccgca
//
