LOCUS       sequence-120919-         727 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  High copy Cas9 expression vector with LEU2 marker for yeast 
            transformation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 727)
  AUTHORS   Laughery MF, Hunter T, Brown A, Hoopes J, Ostbye T, Shumaker T, 
            Wyrick JJ
  TITLE     New vectors for simple and streamlined CRISPR-Cas9 genome editing in
            Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2015 Dec;32(12):711-20. doi: 10.1002/yea.3098. Epub 2015 Sep 
            21.
  PUBMED    26305040
REFERENCE   2  (bases 1 to 727)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/yea.3098"; 
            journalName: "Yeast"; date: "2015-12"; volume: "32"; issue: "12"; 
            pages: "711-20"
COMMENT     Sequence Label: pJH001
FEATURES             Location/Qualifiers
     source          1..727
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(47..67)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 taaatcataa gaaattcgcc tcgagagtct aggatccgga actacctacc ttgcgctttt
       61 tcttgggagc tccctcatcc cctccgagct gtgagaggtc aatccgagtt tcgtaaagtc
      121 cggtgatgct ctggtggatg agggtggcgt ccagcacttc tttggtggag gtatagcgtt
      181 tccggtcgat ggtggtgtcg aagtacttga atgcggcagg ggcaccaaga ttggtgaggg
      241 tgaacaggtg gatgatattc tcagcctgct cccgaattgg tttgtctctg tgcttattgt
      301 atgcgctaag gactttatcg aggttggcat cagcgagaat gacgcgttta gaaaactcgg
      361 aaatctgctc aatgatctcg tccagataat gcttatgctg ctccacaaac agttgctttt
      421 gctcgttgtc ctcggggctc cccttcagct tctcgtaatg gcttgcaagg tagaggaaat
      481 tcacgtactt ggaggggaga gccagctcat tacccttctg aagttcgcca gcggaggcga
      541 gcatccgctt ccgcccattc tccagttcga acaggctata ctttgggagc ttgatgataa
      601 ggtccttctt gacttccttg taacctttgg cttcgagaaa gtcaattggg ttcttctcga
      661 aggagctgcg ctccataatg gtgataccca gcagttcctt cacgctcttg agcttcttag
      721 actttcc
//
