LOCUS       sequence-120922-         845 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Transgenesis vector consisting of a 1.6-kb fragment of MyHC1 
            promoter region upstream of the start codon and an mCherry reporter 
            gene, flanked by inverted binding sites of meganuclease I-SceI.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 845)
  AUTHORS   Renfer E, Amon-Hassenzahl A, Steinmetz PR, Technau U
  TITLE     A muscle-specific transgenic reporter line of the sea anemone, 
            Nematostella vectensis.
  JOURNAL   Proc Natl Acad Sci U S A. 2010 Jan 5;107(1):104-8. doi: 
            10.1073/pnas.0909148107. Epub 2009 Dec 14.
  PUBMED    20018670
REFERENCE   2  (bases 1 to 845)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.0909148107"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2010-01-5- 5"; volume: "107"; issue: "1"; pages: "104-8"
COMMENT     Sequence Label: pNvT-MHC::mCh
FEATURES             Location/Qualifiers
     source          1..845
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    363..484
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(400..419)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     454..473
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(585..604)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(586..604)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(611..628)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(611..627)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(620..642)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 cgaggacggc gccctgaagg gcgagatcaa gcagaggctg aagctgaagg acggcggcca
       61 ctacgacgct gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc
      121 ctacaacgtc aacatcaagt tggacatcac ctcccacaac gaggactaca ccatcgtgga
      181 acagtacgaa cgcgccgagg gccgccactc caccggcggc atggacgagc tgtacaagta
      241 acctgcaggg actctagatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt
      301 taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg
      361 ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
      421 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
      481 cttaaagggc cggccattac cctgttatcc ctatcaaggg cgaattctgc agatatccat
      541 cacactggcg gccgctcgag catgcatcta gagggcccaa ttcgccctat agtgagtcgt
      601 attacaattc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc
      661 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc
      721 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggacg cgccctgtag
      781 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
      841 cgccc
//
