LOCUS       sequence-120932-         867 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9nlsHis6 Bacterial Expression construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Fu BX, Hansen LL, Artiles KL, Nonet ML, Fire AZ
  TITLE     Landscape of target:guide homology effects on Cas9-mediated 
            cleavage.
  JOURNAL   Nucleic Acids Res. 2014 Dec 16;42(22):13778-87. doi: 
            10.1093/nar/gku1102. Epub 2014 Nov 15.
  PUBMED    25399416
REFERENCE   2  (bases 1 to 867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gku1102"; journalName: "Nucleic Acids Res"; date: 
            "2014-12-16- 16"; volume: "42"; issue: "22"; pages: "13778-87"
COMMENT     Sequence Label: pHO4d-Cas9
FEATURES             Location/Qualifiers
     source          1..867
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 tgtttaactt taagaaggag atataccatg gataagaaat actcaatagg cttagatatc
       61 ggcacaaata gcgtcggatg ggcggtgatc actgatgaat ataaggttcc gtctaaaaag
      121 ttcaaggttc tgggaaatac agaccgccac agtatcaaaa aaaatcttat aggggctctt
      181 ttatttgaca gtggagagac agcggaagcg actcgtctca aacggacagc tcgtagaagg
      241 tatacacgtc ggaagaatcg tatttgttat ctacaggaga ttttttcaaa tgagatggcg
      301 aaagtagatg atagtttctt tcatcgactt gaagagtctt ttttggtgga agaagacaag
      361 aagcatgaac gtcatcctat ttttggaaat atagtagatg aagttgctta tcatgagaaa
      421 tatccaacta tctatcatct gcgaaaaaaa ttggtagatt ctactgataa agcggatttg
      481 cgcttaatct atttggcctt agcgcatatg attaagtttc gtggtcattt tttgattgag
      541 ggagatttaa atcctgataa tagtgatgtg gacaaactat ttatccagtt ggtacaaacc
      601 tacaatcaat tatttgaaga aaaccctatt aacgcaagtg gagtagatgc taaagcgatt
      661 ctttctgcac gattgagtaa atcaagacga ttagaaaatc tcattgctca gctccccggt
      721 gagaagaaaa atggcttatt tgggaatctc attgctttgt cattgggttt gacccctaat
      781 tttaaatcaa attttgattt ggcagaagat gctaaattac agctttcaaa agatacttac
      841 gatgatgatt tagataattt attggcg
//
