LOCUS       sequence-120933-         786 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of a photoactivatable fluorescent calcium 
            reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 786)
  AUTHORS   Berlin S, Carroll EC, Newman ZL, Okada HO, Quinn CM, Kallman B, 
            Rockwell NC, Martin SS, Lagarias JC, Isacoff EY
  TITLE     Photoactivatable genetically encoded calcium indicators for targeted
            neuronal imaging.
  JOURNAL   Nat Methods. 2015 Jul 13. doi: 10.1038/nmeth.3480.
  PUBMED    26167640
REFERENCE   2  (bases 1 to 786)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Jul 13. doi: 10.1038/nmeth.3480."
COMMENT     Sequence Label: pRSET-triple mutation-pa-GCaMP6f
FEATURES             Location/Qualifiers
     source          1..786
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             40..57
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             61..93
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(97..105,106..120)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     regulatory      127..136
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             148..204
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
     primer_bind     417..438
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(544..565)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 ttttgtttac tttaagaagg agatagatct atgggttctc atcatcatca tcatcatggt
       61 atggctagca tgactggtgg acagcaaatg ggtcgggatc tgtacgacga tgacgataag
      121 gatctcgcca ccatggtcga ctcatcacgt cgtaagtgga ataagacagg tcacgcagtc
      181 agagctatag gtcggctgag ctcactcgag aacgtctata tcaaggccga caagcagaag
      241 aacggcatca aggcgaactt caagatccgc cacaacatcg aggacggcgg cgtgcagctc
      301 gcctaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac
      361 cactacctga gccatcagtc caaactttcg aaagacccca acgagaagcg cgatcacatg
      421 gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag
      481 ggcggtaccg gagggagcat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
      541 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggt
      601 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
      661 cccgtgccct ggcccaccct cgtgaccacc ttcagctacg gcgtgcagtg cttcagccgc
      721 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacatc
      781 caggag
//
