LOCUS       sequence-120941-         880 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Wild-type OmpA (N-terminal domain) with an HA tag in the 4th 
            external loop.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Stapleton JA, Whitehead TA, Nanda V
  TITLE     Computational redesign of the lipid-facing surface of the outer 
            membrane protein OmpA.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
  PUBMED    26199411
REFERENCE   2  (bases 1 to 880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2015 Jul 21. pii: 201501836."
COMMENT     Sequence Label: pJexpress414_OmpA171_WT
FEATURES             Location/Qualifiers
     source          1..880
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(214..261)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(275..292)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(293..310)
                     /codon_start=1
                     /product="tetracysteine peptide that binds biarsenical 
                     labeling reagents"
                     /label=tetracysteine tag
                     /translation="CCPGCC"
     CDS             complement(362..388)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 cggcagtagc gcggtggtcc cacctgaccc catgccgaac tcagaagtga aacgccgtag
       61 cgccgatggt agtgtgggga ctccccatgc gagagtaggg aactgccagg catcaaataa
      121 aacgaaaggc tcagtcgaaa gactgggcct ttcgcccggg ctaattatgg ggtgtcgccc
      181 ttaggtacga actcgattga cgcgtctcag gggcaaaaaa cccctcaaga cccgtttaga
      241 ggccccaagg ggttatgcta gggggaagct tttagtgatg gtggtgatgg tgacagcaac
      301 cagggcaaca accgaaacgg taggaaacac ccaggctcag catgccgtta tccggacgag
      361 tcgcgtaatc tggaacatcg tatgggtagt caccgatgtt gttggtccac tggtattcca
      421 gacgggtagc gatctccgga gtgatcgcgt actcaacacc gccagcgaag accggagaaa
      481 cgccggtatc gtggttttta ccataaacgt tggatttagt atctgcacgc cataccatgc
      541 cacccagacg agtatagata tccaggtcgt cggtgatcgg gtaacccagt ttagcggtca
      601 gttgaacgcc ctgagccttg tatgcaccgt tttcaacgct gcctttgtac ggcatacgac
      661 cgagccagtc gtaacccatt tcaaagccaa cgtacgggtt cacctggtaa ccaccaaaag
      721 caccagcgcc cagctggttc tcgtgggtcg ggccattatt attgatgaaa ccagtgtcgt
      781 ggtactggga ccagcccagt ttagcaccag tataccaggt gttgtcgttc ggcgcggcct
      841 gcgctacggt agcgaaacca gccagtgcca ctgcgatggc
//
