LOCUS       sequence-120944-         832 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  OmpA redesign hybrid with wild-type strands 3478.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Stapleton JA, Whitehead TA, Nanda V
  TITLE     Computational redesign of the lipid-facing surface of the outer 
            membrane protein OmpA.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
  PUBMED    26199411
REFERENCE   2  (bases 1 to 832)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2015 Jul 21. pii: 201501836."
COMMENT     Sequence Label: pJexpress414_OmpA171_H3478
FEATURES             Location/Qualifiers
     source          1..832
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     sig_peptide     21..83
                     /codon_start=1
                     /product="signal peptide from the E. coli outer membrane 
                     protein OmpA"
                     /label=OmpA signal peptide
                     /translation="MKKTAIAIAVALAGFATVAQA"
     CDS             531..557
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             609..626
                     /codon_start=1
                     /product="tetracysteine peptide that binds biarsenical 
                     labeling reagents"
                     /label=tetracysteine tag
                     /translation="CCPGCC"
     CDS             627..644
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      658..705
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 acttttagga gataaaacat atgaaaaaga ccgcaatcgc catcgcagtg gcactggctg
       61 gtttcgctac cgtagcgcag gccgcgccga acgacaacac cttttacctg ggtttgaaga
      121 tgggtatttc ccagtaccac gacactggtt tcatcaataa taatggcccg acccacgaga
      181 accagcaggg tatcggtgcc tttgcaggca tccaggtgaa cccgtacgtt ggctttgaaa
      241 tgggttacga ctggctcggt cgtatgccgt acaaaggcag cgttgaaaac ggtgcataca
      301 aggctcaggg cgttcaactg accgctaaac tgggttaccc gatcaccgac gacgttgacg
      361 tgtacctgcg tctgggcatc atggtatggc gtgcagatac taaatccaac gtttatggta
      421 aaaaccacga taccggcgtt tctttcgtca tggcgctggg tagcgaacat gcgatcactc
      481 cggagatcgc tacccgtctg gaataccagt ggaccaacaa catcggtgac tacccatacg
      541 atgttccaga ttacgcgact cgtccggata acggcatgct gagcctgggt gtttcctacc
      601 gtttcggttg ttgccctggt tgctgtcacc atcaccacca tcactaaaag cttcccccta
      661 gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgcccct gagacgcgtc
      721 aatcgagttc gtacctaagg gcgacacccc ataattagcc cgggcgaaag gcccagtctt
      781 tcgactgagc ctttcgtttt atttgatgcc tggcagttcc ctactctcgc at
//
