LOCUS       sequence-120945-         833 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Conservative version of OmpA Redesign 4 with Y129H mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Stapleton JA, Whitehead TA, Nanda V
  TITLE     Computational redesign of the lipid-facing surface of the outer 
            membrane protein OmpA.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
  PUBMED    26199411
REFERENCE   2  (bases 1 to 833)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2015 Jul 21. pii: 201501836."
COMMENT     Sequence Label: pJexpress414_OmpA171_R4cons_Y129H
FEATURES             Location/Qualifiers
     source          1..833
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     sig_peptide     21..83
                     /codon_start=1
                     /product="signal peptide from the E. coli outer membrane 
                     protein OmpA"
                     /label=OmpA signal peptide
                     /translation="MKKTAIAIAVALAGFATVAQA"
     CDS             597..614
                     /codon_start=1
                     /product="tetracysteine peptide that binds biarsenical 
                     labeling reagents"
                     /label=tetracysteine tag
                     /translation="CCPGCC"
     CDS             615..632
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      646..693
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 acttttagga gataaaacat atgaaaaaga ccgcaatcgc catcgcagtg gcactggctg
       61 gtttcgctac cgtagcgcag gccgcgccga aagacaacac cttctatctc ggcctgaaaa
      121 tggggatcag ccagtatcac gatacgggat ttattaacaa taatggtccg acgcatgaaa
      181 atcagctggg gatcggcgca tttggaggta tccaggtcaa tccctacgtt ggcctggaac
      241 tcggtgtcga cgtgctgggt cgtatgccct acaaaggcag tgtggaaaat ggggcgtata
      301 aagcacaagg gtatcaagca actcttaaga tgggcgtgcc gattaccgat gacgtggacg
      361 tatatctgcg tttaggcggt atggtatggc gtgcagatac caaatctaat gtttatggca
      421 aaaaccatga tactggcgtt agcccagtga tggcaggtgg ctctgaacat gcaattaccc
      481 ctgaaatcgc aatgcgcctc gaaacacaat ggactaacaa tattggtgat gcgcacacca
      541 ttggtacccg tccggacaat ctgatgctgt ctctgggtct gtcctaccgt ttcggttgtt
      601 gccctggttg ctgtcaccat caccaccatc actaaaagct tccccctagc ataacccctt
      661 ggggcctcta aacgggtctt gaggggtttt ttgcccctga gacgcgtcaa tcgagttcgt
      721 acctaagggc gacaccccat aattagcccg ggcgaaaggc ccagtctttc gactgagcct
      781 ttcgttttat ttgatgcctg gcagttccct actctcgcat ggggagtccc cac
//
