LOCUS       sequence-120947-         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Conservative version of OmpA Redesign 4 with Y129H mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Stapleton JA, Whitehead TA, Nanda V
  TITLE     Computational redesign of the lipid-facing surface of the outer 
            membrane protein OmpA.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
  PUBMED    26199411
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2015 Jul 21. pii: 201501836."
COMMENT     Sequence Label: pJexpress414_OmpA171_R4cons_Y129H
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(204..251)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(265..282)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(283..300)
                     /codon_start=1
                     /product="tetracysteine peptide that binds biarsenical 
                     labeling reagents"
                     /label=tetracysteine tag
                     /translation="CCPGCC"
ORIGIN
        1 gcggtggtcc cacctgaccc catgccgaac tcagaagtga aacgccgtag cgccgatggt
       61 agtgtgggga ctccccatgc gagagtaggg aactgccagg catcaaataa aacgaaaggc
      121 tcagtcgaaa gactgggcct ttcgcccggg ctaattatgg ggtgtcgccc ttaggtacga
      181 actcgattga cgcgtctcag gggcaaaaaa cccctcaaga cccgtttaga ggccccaagg
      241 ggttatgcta gggggaagct tttagtgatg gtggtgatgg tgacagcaac cagggcaaca
      301 accgaaacgg taggacagac ccagagacag catcagattg tccggacggg taccaatggt
      361 gtgcgcatca ccaatattgt tagtccattg tgtttcgagg cgcattgcga tttcaggggt
      421 aattgcatgt tcagagccac ctgccatcac tgggctaacg ccagtatcat ggtttttgcc
      481 ataaacatta gatttggtat ctgcacgcca taccataccg cctaaacgca gatatacgtc
      541 cacgtcatcg gtaatcggca cgcccatctt aagagttgct tgataccctt gtgctttata
      601 cgccccattt tccacactgc ctttgtaggg catacgaccc agcacgtcga caccgagttc
      661 caggccaacg tagggattga cctggatacc tccaaatgcg ccgatcccca gctgattttc
      721 atgcgtcgga ccattattgt taataaatcc cgtatcgtga tactggctga tccccatttt
      781 caggccgaga tagaaggtgt tgtctttcgg cgcggcctgc gctacggtag cgaaaccagc
      841 cagtgccact
//
