LOCUS       sequence-120958-         792 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  luciferase reporter for hA33 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 792)
  AUTHORS   Johnstone CN, White SJ, Tebbutt NC, Clay FJ, Ernst M, Biggs WH, 
            Viars CS, Czekay S, Arden KC, Heath JK
  TITLE     Analysis of the regulation of the A33 antigen gene reveals 
            intestine-specific mechanisms of gene expression.
  JOURNAL   J Biol Chem. 2002 Sep 13;277(37):34531-9. Epub 2002 Jul 11.
  PUBMED    12114523
REFERENCE   2  (bases 1 to 792)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2002-09-13"; volume: "277(37)"; pages: "34531-9. Epub
            2002 Jul 11"
COMMENT     Sequence Label: 0.44 hA33 luciferase
FEATURES             Location/Qualifiers
     source          1..792
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      35..44
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    516..607
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(548..567)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    621..669
                     /note="synthetic polyadenylation signal"
ORIGIN
        1 ggcgccgggc ctttctttat gtttttggcg tcttccatgg tggctttacc aacagtaccg
       61 gaatgccaag cttacttaga tcgcagatct cgagcactgg cagcctccat acaggtctga
      121 gctgtctggt taaagctaca gcagcttctg gcctggccca actggaggtc cctgcaattt
      181 tccctcttct cacccacctc tgcccagcct cagctggatt ggcaggtgag ctcctcagct
      241 ggattggcag gtgagctggg gctgggctca tgtgtcacct actccaaagg tcccgagagg
      301 agtcttccct acttccctca agttcccctt aaaccagcca cctcccctgc cttctcccta
      361 cttagcaggt gtacaaagtc ctagactgct cacaaagggg taggcctctc ttataactga
      421 caggaaataa ataataaaaa gggaaatgca ctcagaggta gggttccaag ctagtaaaag
      481 aagggtgttg cccatatttg ctggtaccta tcgatagaga aatgttctgg cacctgcact
      541 tgcactgggg acagcctatt ttgctagttt gttttgtttc gttttgtttt gatggagagc
      601 gtatgttagt actatcgatt cacacaaaaa accaacacac agatgtaatg aaaataaaga
      661 tattttattg cggccgctcc aagtacctcc cgtaccttaa tattacttac ttatcatggt
      721 agcttgggct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
      781 agcctgaatg gc
//
