LOCUS       sequence-120960-         788 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  luciferase reporter for hA33 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 788)
  AUTHORS   Johnstone CN, White SJ, Tebbutt NC, Clay FJ, Ernst M, Biggs WH, 
            Viars CS, Czekay S, Arden KC, Heath JK
  TITLE     Analysis of the regulation of the A33 antigen gene reveals 
            intestine-specific mechanisms of gene expression.
  JOURNAL   J Biol Chem. 2002 Sep 13;277(37):34531-9. Epub 2002 Jul 11.
  PUBMED    12114523
REFERENCE   2  (bases 1 to 788)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2002-09-13"; volume: "277(37)"; pages: "34531-9. Epub
            2002 Jul 11"
COMMENT     Sequence Label: 0.42 hA33 luciferase
FEATURES             Location/Qualifiers
     source          1..788
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      33..42
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    493..584
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(525..544)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    598..646
                     /note="synthetic polyadenylation signal"
ORIGIN
        1 cgccgggcct ttctttatgt ttttggcgtc ttccatggtg gctttaccaa cagtaccgga
       61 atgccaagct tacttagatc gcagatctcg agcactggca gcctccatac aggtctgagc
      121 tgtctggtta aagctacagc agcttctggc ctggcccaac tggaggtccc tgcaattttc
      181 cctcttctca cccacctctg cccagcctca gctggattgg caggtgagct cctcagctgg
      241 attggcaggt gagctggggc tgggctcatg tgtcacctac tccaaaggtc ccgagaggag
      301 tcttccctac ttccctcaag ttccccttaa accagccacc tcccctgcct tctccctact
      361 tagcaggtgt acaaagtcct agactgctca caaaggggta ggcctctctt ataactgaca
      421 ggaaataaat aataaaaagg gaaatgcact cagaggtagg gttccaagct agtaaaagag
      481 gtacctatcg atagagaaat gttctggcac ctgcacttgc actggggaca gcctattttg
      541 ctagtttgtt ttgtttcgtt ttgttttgat ggagagcgta tgttagtact atcgattcac
      601 acaaaaaacc aacacacaga tgtaatgaaa ataaagatat tttattgcgg ccgctccaag
      661 tacctcccgt accttaatat tacttactta tcatggtagc ttgggctggc gtaatagcga
      721 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcaaat
      781 tgtaagcg
//
