LOCUS       sequence-120966-         737 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  luciferase reporter for hA33 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 737)
  AUTHORS   Johnstone CN, White SJ, Tebbutt NC, Clay FJ, Ernst M, Biggs WH, 
            Viars CS, Czekay S, Arden KC, Heath JK
  TITLE     Analysis of the regulation of the A33 antigen gene reveals 
            intestine-specific mechanisms of gene expression.
  JOURNAL   J Biol Chem. 2002 Sep 13;277(37):34531-9. Epub 2002 Jul 11.
  PUBMED    12114523
REFERENCE   2  (bases 1 to 737)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2002-09-13"; volume: "277(37)"; pages: "34531-9. Epub
            2002 Jul 11"
COMMENT     Sequence Label: 0.36 hA33 luciferase
FEATURES             Location/Qualifiers
     source          1..737
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      34..43
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    435..526
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(467..486)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    540..588
                     /note="synthetic polyadenylation signal"
ORIGIN
        1 gcgccgggcc tttctttatg tttttggcgt cttccatggt ggctttacca acagtaccgg
       61 aatgccaagc ttacttagat cgcagatctc gagcactggc agcctccata caggtctgag
      121 ctgtctggtt aaagctacag cagcttctgg cctggcccaa ctggaggtcc ctgcaatttt
      181 ccctcttctc acccacctct gcccagcctc agctggattg gcaggtgagc tcctcagctg
      241 gattggcagg tgagctgggg ctgggctcat gtgtcaccta ctccaaaggt cccgagagga
      301 gtcttcccta cttccctcaa gttcccctta aaccagccac ctcccctgcc ttctccctac
      361 ttagcaggtg tacaaagtcc tagactgctc acaaaggggt aggcctctct tataactgac
      421 aggtacctat cgatagagaa atgttctggc acctgcactt gcactgggga cagcctattt
      481 tgctagtttg ttttgtttcg ttttgttttg atggagagcg tatgttagta ctatcgattc
      541 acacaaaaaa ccaacacaca gatgtaatga aaataaagat attttattgc ggccgctcca
      601 agtacctccc gtaccttaat attacttact tatcatggta gcttgggctg gcgtaatagc
      661 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggcaa
      721 attgtaagcg ttaatat
//
