LOCUS       sequence-120970-        1093 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  RhoC biosensor single chain; mCerulean-tagged ROCK-RBD, 
            mVenus-tagged RhoC.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1093)
  AUTHORS   Zawistowski JS, Sabouri-Ghomi M, Danuser G, Hahn KM, Hodgson L
  TITLE     A RhoC biosensor reveals differences in the activation kinetics of 
            RhoA and RhoC in migrating cells.
  JOURNAL   PLoS One. 2013 Nov 5;8(11):e79877. doi: 
            10.1371/journal.pone.0079877. eCollection 2013.
  PUBMED    24224016
REFERENCE   2  (bases 1 to 1093)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-11-5"; volume: "8(11):e79877. doi"; pages: " 
            10.1371/journal.pone.0079877. eCollection 2013"
COMMENT     Sequence Label: RhoC FLARE.sc mCer, mVenus - wt
FEATURES             Location/Qualifiers
     source          1..1093
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(74..97)
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             complement(695..946)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(737..758)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ttgtgagcca gggcattggc cacaccagcc accaccttct gataggcagc ctgcacctga
       61 ggttaatcac ttagtgatgg tgatggtgat ggtggtgctc gagtcagaga atgggacagc
      121 ccctccgacg cttgttcttg cggacctgga ggccagcccg agtggccatc tcaaacacct
      181 cccgcactcc ctccttggtc ttggctgagc actcaaggta gccaaaggca ctgatccggt
      241 tcgccatgtc ccggccttcc tcagaccgaa cgggctcctg cttcatcttg gccagctctc
      301 tcctggtgtg ctcgtcttgc ctcaggtcct tcttattccc caccaggatg atgggcacgt
      361 tggggcagaa gtgcttcacc tctggggtcc acttctcagg aatgttttcc aggctgtcag
      421 ggctgtcgat ggagaagcac atgaggatga catcagtgtc cgggtaggag agaggccgca
      481 gtcgatcata gtcttcctgc cctgctgtgt cccacagagc cagctccacc tgcttgccgt
      541 ccacctcaat gtccgcaata tagttctcaa agacagtagg gacgtagacc tccggaaact
      601 gatccttgct gaagacgatg aggaggcagg tcttcccaca ggcaccatcc ccaacgatca
      661 ccagtttctt ccggatggca gccattgtga attccttgta cagctcgtcc atgccgagag
      721 tgatcccggc ggcggtcacg aactccagca ggaccatgtg atcgcgcttc tcgttggggt
      781 ctttgctcag tttggactgg tagctcaggt agtggttgtc gggcagcagc acggggccgt
      841 cgccgatggg ggtgttctgc tggtagtggt cggcgagctg cacgccgccg tcctcgatgt
      901 tgtggcggat cttgaagttt gccttgatgc cgttcttctg cttgtcggcc atgatataga
      961 cgttgtggct gttgtagttg tactccagct tgtgccccag gatgttgccg tcctccttga
     1021 agtcgatgcc cttcagctcg atgcggttca ccagggtgtc gccctcgaac ttcacctcgg
     1081 cgcgggtctt gta
//
