LOCUS       sequence-120973-         726 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  luciferase reporter for hA33 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 726)
  AUTHORS   Johnstone CN, White SJ, Tebbutt NC, Clay FJ, Ernst M, Biggs WH, 
            Viars CS, Czekay S, Arden KC, Heath JK
  TITLE     Analysis of the regulation of the A33 antigen gene reveals 
            intestine-specific mechanisms of gene expression.
  JOURNAL   J Biol Chem. 2002 Sep 13;277(37):34531-9. Epub 2002 Jul 11.
  PUBMED    12114523
REFERENCE   2  (bases 1 to 726)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2002-09-13"; volume: "277(37)"; pages: "34531-9. Epub
            2002 Jul 11"
COMMENT     Sequence Label: 0.38 hA33 luciferase
FEATURES             Location/Qualifiers
     source          1..726
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      35..44
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    456..547
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(488..507)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    561..609
                     /note="synthetic polyadenylation signal"
ORIGIN
        1 ggcgccgggc ctttctttat gtttttggcg tcttccatgg tggctttacc aacagctacc
       61 ggaatgccaa gcttacttag atcgcagatc tcgagcactg gcagcctcca tacaggtctg
      121 agctgtctgg ttaaagctac agcagcttct ggcctggccc aactggaggt ccctgcaatt
      181 ttccctcttc tcacccacct ctgcccagcc tcagctggat tggcaggtga gctcctcagc
      241 tggattggca ggtgagctgg ggctgggctc atgtgtcacc tactccaaag gtcccgagag
      301 gagtcttccc tacttccctc aagttcccct taaaccagcc acctcccctg ccttctccct
      361 acttagcagg tgtacaaagt cctagactgc tcacaaaggg gtaggcctct cttataactg
      421 acaggaaata aataataaaa agggtaccta tcgatagaga aatgttctgg cacctgcact
      481 tgcactgggg acagcctatt ttgctagttt gttttgtttc gttttgtttt gatggagagc
      541 gtatgttagt actatcgatt cacacaaaaa accaacacac agatgtaatg aaaataaaga
      601 tattttattg cggccgctcc aagtacctcc cgtaccttaa tattacttac ttatcatggt
      661 agcttgggct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
      721 agcctg
//
