LOCUS       sequence-120979-         786 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  luciferase reporter for hA33 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 786)
  AUTHORS   Johnstone CN, White SJ, Tebbutt NC, Clay FJ, Ernst M, Biggs WH, 
            Viars CS, Czekay S, Arden KC, Heath JK
  TITLE     Analysis of the regulation of the A33 antigen gene reveals 
            intestine-specific mechanisms of gene expression.
  JOURNAL   J Biol Chem. 2002 Sep 13;277(37):34531-9. Epub 2002 Jul 11.
  PUBMED    12114523
REFERENCE   2  (bases 1 to 786)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2002-09-13"; volume: "277(37)"; pages: "34531-9. Epub
            2002 Jul 11"
COMMENT     Sequence Label: 0.22 hA33 luciferase
FEATURES             Location/Qualifiers
     source          1..786
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      35..44
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    295..386
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(327..346)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    400..448
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(716..737)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ggcgccgggc ctttctttat gtttttggcg tcttccatgg tggctttacc aacagtaccg
       61 gaatgccaag cttacttaga tcgcagatct cgagcactag cagcctccat acaggtctga
      121 gctgtctggt taaagctaca gcagcttctg gcctggccca actggaggtc cctgcaattt
      181 tccctcttct cacccacctc tgcccagcct cagctggatt ggcaggtgag ctcctcagct
      241 ggattggcag gtgagctggg gctgggctca tgtgtcacct aggtacctat cgatagagaa
      301 atgttctggc acctgcactt gcactgggga cagcctattt tgctagtttg ttttgtttcg
      361 ttttgttttg atggagagcg tatgttagta ctatcgattc acacaaaaaa ccaacacaca
      421 gatgtaatga aaataaagat attttattgc ggccgctcca agtacctccc gtaccttaat
      481 attacttact tatcatggta gcttgggctg gcgtaatagc gaagaggccc gcaccgatcg
      541 cccttcccaa cagttgcgca gcctgaatgg cgaatggcaa attgtaagcg ttaatatttt
      601 gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat
      661 cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt
      721 ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt
      781 ctatca
//
