LOCUS       sequence-121012-         735 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Cas9 and contains guide RNA expression cassette with 
            BclI-SwaI cloning sites for guide sequence cloning; Contains URA3 
            marker for yeast transformation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 735)
  AUTHORS   Laughery MF, Hunter T, Brown A, Hoopes J, Ostbye T, Shumaker T, 
            Wyrick JJ
  TITLE     New vectors for simple and streamlined CRISPR-Cas9 genome editing in
            Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2015 Dec;32(12):711-20. doi: 10.1002/yea.3098. Epub 2015 Sep 
            21.
  PUBMED    26305040
REFERENCE   2  (bases 1 to 735)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/yea.3098"; 
            journalName: "Yeast"; date: "2015-12"; volume: "32"; issue: "12"; 
            pages: "711-20"
COMMENT     Sequence Label: pML104
FEATURES             Location/Qualifiers
     source          1..735
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      110..119
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ttctactttt atagttagtc ttttttttag ttttaaaaca ccaagaactt agtttcgaat
       61 aaacacacat aaacaaacaa aagatctgat atggatcgaa ttagatctcg ccaccatgga
      121 caagaagtat tctatcggac tggacatcgg gactaatagc gtcgggtggg ccgtcatcac
      181 tgacgagtac aaggtgccct ctaagaagtt caaggtgctc gggaacaccg accggcattc
      241 catcaagaaa aatctgatcg gagctctcct ctttgattca ggggagaccg ctgaagcaac
      301 ccgcctcaag cggactgcta gacggcggta caccaggagg aagaaccgga tttgttacct
      361 tcaagagata ttctccaacg aaatggcaaa ggtcgacgac agcttcttcc ataggctgga
      421 agaatcattc ctcgtggaag aggataagaa gcatgaacgg catcccatct tcggtaatat
      481 cgtcgacgag gtggcctatc acgagaaata cccaaccatc taccatcttc gcaaaaagct
      541 ggtggactca accgacaagg cagacctccg gcttatctac ctggccctgg cccacatgat
      601 taagttcaga ggccacttcc tgatcgaggg cgacctcaat cctgacaata gcgatgtgga
      661 taaactgttc atccagctgg tgcagactta caaccagctc tttgaagaga accccatcaa
      721 tgcaagcgga gtcga
//
