LOCUS       sequence-121014-         729 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Cas9 and contains guide RNA expression cassette with 
            BclI-SwaI cloning sites for guide sequence cloning; Contains URA3 
            marker for yeast transformation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 729)
  AUTHORS   Laughery MF, Hunter T, Brown A, Hoopes J, Ostbye T, Shumaker T, 
            Wyrick JJ
  TITLE     New vectors for simple and streamlined CRISPR-Cas9 genome editing in
            Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2015 Dec;32(12):711-20. doi: 10.1002/yea.3098. Epub 2015 Sep 
            21.
  PUBMED    26305040
REFERENCE   2  (bases 1 to 729)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/yea.3098"; 
            journalName: "Yeast"; date: "2015-12"; volume: "32"; issue: "12"; 
            pages: "711-20"
COMMENT     Sequence Label: pML104
FEATURES             Location/Qualifiers
     source          1..729
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      343..362
                     /label=SUP4 terminator
                     /note="transcription terminator for the S. cerevisiae SUP4 
                     tRNA gene"
     terminator      complement(480..667)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     588..612
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             complement(695..715)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 aagataatgt atgattatgc tttcactcat atttatacag aaacttgatg ttttctttcg
       61 agtatataca aggtgattac atgtacgttt gaagtacaac tctagatttt gtagtgccct
      121 cttgggctag cggtaaaggt gcgcattttt tcacacccta caatgttctg ttcaaaagat
      181 tttggtcaaa cgctgtagaa gtgaaagttg gtgcgcatgt ttcggcgttc gaaacttctc
      241 cgcagtgaaa gataaatgat cagaatcgat gcatttaaat agcaagttaa aataaggcta
      301 gtccgttatc aacttgaaaa agtggcaccg agtcggtggt gctttttttg ttttttatgt
      361 cttcgagtca tgtaattagt tataagcatg tgagctaaga cactgtaatt gccaatctaa
      421 acgataccac ggccgctcta gagaaatggg gagcgatttg caggcatttg ctcggcatgc
      481 cggtagaggt gtggtcaata agagcgacct catgctatac ctgagaaagc aacctgacct
      541 acaggaaaga gttactcaag aataagaatt ttcgttttaa aacctaagag tcactttaaa
      601 atttgtatac acttattttt tttataactt atttaataat aaaaatcata aatcataaga
      661 aattcgcctc gagagtctag gatccggaac tacctacctt gcgctttttc ttgggagctc
      721 cctcatccc
//
