LOCUS       sequence-121016-         729 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Cas9 and contains guide RNA expression cassette with 
            BclI-SwaI cloning sites for guide sequence cloning; Contains LEU2 
            marker for yeast transformation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 729)
  AUTHORS   Laughery MF, Hunter T, Brown A, Hoopes J, Ostbye T, Shumaker T, 
            Wyrick JJ
  TITLE     New vectors for simple and streamlined CRISPR-Cas9 genome editing in
            Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2015 Dec;32(12):711-20. doi: 10.1002/yea.3098. Epub 2015 Sep 
            21.
  PUBMED    26305040
REFERENCE   2  (bases 1 to 729)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/yea.3098"; 
            journalName: "Yeast"; date: "2015-12"; volume: "32"; issue: "12"; 
            pages: "711-20"
COMMENT     Sequence Label: pML107
FEATURES             Location/Qualifiers
     source          1..729
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      105..114
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 cttttatagt tagtcttttt tttagtttta aaacaccaag aacttagttt cgaataaaca
       61 cacataaaca aacaaaagat ctgatatgga tcgaattaga tctcgccacc atggacaaga
      121 agtattctat cggactggac atcgggacta atagcgtcgg gtgggccgtc atcactgacg
      181 agtacaaggt gccctctaag aagttcaagg tgctcgggaa caccgaccgg cattccatca
      241 agaaaaatct gatcggagct ctcctctttg attcagggga gaccgctgaa gcaacccgcc
      301 tcaagcggac tgctagacgg cggtacacca ggaggaagaa ccggatttgt taccttcaag
      361 agatattctc caacgaaatg gcaaaggtcg acgacagctt cttccatagg ctggaagaat
      421 cattcctcgt ggaagaggat aagaagcatg aacggcatcc catcttcggt aatatcgtcg
      481 acgaggtggc ctatcacgag aaatacccaa ccatctacca tcttcgcaaa aagctggtgg
      541 actcaaccga caaggcagac ctccggctta tctacctggc cctggcccac atgattaagt
      601 tcagaggcca cttcctgatc gagggcgacc tcaatcctga caatagcgat gtggataaac
      661 tgttcatcca gctggtgcag acttacaacc agctctttga agagaacccc atcaatgcaa
      721 gcggagtcg
//
