LOCUS       sequence-121018-         636 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Cas9 and contains guide RNA expression cassette with 
            BclI-SwaI cloning sites for guide sequence cloning; Contains LEU2 
            marker for yeast transformation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 636)
  AUTHORS   Laughery MF, Hunter T, Brown A, Hoopes J, Ostbye T, Shumaker T, 
            Wyrick JJ
  TITLE     New vectors for simple and streamlined CRISPR-Cas9 genome editing in
            Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2015 Dec;32(12):711-20. doi: 10.1002/yea.3098. Epub 2015 Sep 
            21.
  PUBMED    26305040
REFERENCE   2  (bases 1 to 636)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/yea.3098"; 
            journalName: "Yeast"; date: "2015-12"; volume: "32"; issue: "12"; 
            pages: "711-20"
COMMENT     Sequence Label: pML107
FEATURES             Location/Qualifiers
     source          1..636
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      343..362
                     /label=SUP4 terminator
                     /note="transcription terminator for the S. cerevisiae SUP4 
                     tRNA gene"
     primer_bind     588..612
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
ORIGIN
        1 aagataatgt atgattatgc tttcactcat atttatacag aaacttgatg ttttctttcg
       61 agtatataca aggtgattac atgtacgttt gaagtacaac tctagatttt gtagtgccct
      121 cttgggctag cggtaaaggt gcgcattttt tcacacccta caatgttctg ttcaaaagat
      181 tttggtcaaa cgctgtagaa gtgaaagttg gtgcgcatgt ttcggcgttc gaaacttctc
      241 cgcagtgaaa gataaatgat cagaatcgat gcatttaaat agcaagttaa aataaggcta
      301 gtccgttatc aacttgaaaa agtggcaccg agtcggtggt gctttttttg ttttttatgt
      361 cttcgagtca tgtaattagt tataagcatg tgagctaaga cactgtaatt gccaatctaa
      421 acgataccac ggccgntcta gagaaatggg gagcgatttg caggcatttg ctcggcatgc
      481 cggtagaggt gtggtcaata agagcgacct catgctatac ctgagaaagc aacctgacct
      541 acaggaaaga gttactcaag aataagaatt ttcgttttaa aacctaagag tcactttaaa
      601 atttgtatac acttattttt tttataactt atttaa
//
