LOCUS       sequence-121022-         928 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Middle entry nuclear localized tdTomato reporter with 6xMT and 2A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 928)
  AUTHORS   Shin M, Beane TJ, Quillien A, Male I, Zhu LJ, Lawson ND
  TITLE     Vegfa signals through ERK to promote angiogenesis, but not artery 
            differentiation.
  JOURNAL   Development. 2016 Oct 15;143(20):3796-3805. Epub 2016 Aug 30.
  PUBMED    27578780
REFERENCE   2  (bases 1 to 928)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development."; date: "2016-10-15"; volume: "143(20)"; pages: 
            "3796-3805. Epub 2016 Aug 30"
COMMENT     Sequence Label: pME-6MTnlstdTomato2A
FEATURES             Location/Qualifiers
     source          1..928
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    11..110
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     regulatory      113..122
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             149..178
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             188..217
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             227..256
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             266..295
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             305..334
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             368..397
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             422..442
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(576..594)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     844..863
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 cctcgggccc caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat
       61 tgatgagcaa tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgccaccat
      121 ggctggatcc catcgattta aagctatgga gcaaaagctc atttctgaag aggacttgaa
      181 tgaaatggag caaaagctca tttctgaaga ggacttgaat gaaatggagc aaaagctcat
      241 ttctgaagag gacttgaatg aaatggagca aaagctcatt tctgaagagg acttgaatga
      301 aatggagcaa aagctcattt ctgaagagga cttgaatgaa atggagagct tgggcgacct
      361 caccatggag caaaagctca tttctgaaga ggacttgaat ttagagatct cgaattccat
      421 gcccaagaag aagaggaagg tgatggtgag caagggcgag gaggtcatca aagagttcat
      481 gcgcttcaag gtgcgcatgg agggctccat gaacggccac gagttcgaga tcgagggcga
      541 gggcgagggc cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggcgg
      601 ccccctgccc ttcgcctggg acatcctgtc cccccagttc atgtacggct ccaaggcgta
      661 cgtgaagcac cccgccgaca tccccgatta caagaagctg tccttccccg agggcttcaa
      721 gtgggagcgc gtgatgaact tcgaggacgg cggtctggtg accgtgaccc aggactcctc
      781 cctgcaggac ggcacgctga tctacaaggt gaagatgcgc ggcaccaact tcccccccga
      841 cggccccgta atgcagaaga agaccatggg ctgggaggcc tccaccgagc gcctgtaccc
      901 ccgcgacggc gtgctgaagg gcgagacc
//
