LOCUS       sequence-121025-        1108 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  optical depolarization of mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1108)
  AUTHORS   Offenhaeusser A
  TITLE     ChR2opt_mKATE
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1108)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pcDNA4_ChR2opt_mKATE
FEATURES             Location/Qualifiers
     source          1..1108
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    18..36
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    39..57
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     67..91
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      182..191
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ctatataagc agagctctcc ctatcagtga tagagatctc cctatcagtg atagagatcg
       61 tcgacgagct cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga
      121 cctccataga agacaccggg accgatccag cctccggact ctagcgttta aacttaagct
      181 tgccaccatg gactacggcg gagccctgag cgccgtgggc agagaactgc tgttcgtgac
      241 caaccccgtg gtggtcaacg gcagcgtgct ggtgcccgag gaccagtgtt actgcgccgg
      301 ctggatcgag agcagaggca caaacggcgc ccagaccgcc tctaacgtgc tgcagtggct
      361 ggccgctggc ttcagcatcc tgctgctgat gttctacgcc taccagacct ggaagtctac
      421 ctgcggctgg gaagagatct acgtgtgcgc catcgagatg gtcaaagtga tcctcgagtt
      481 cttcttcgaa tttaaaaacc ccagcatgct gtacctggcc accggccaca gagtccagtg
      541 gctgagatac gccgagtggc tgctgacctg ccccgtgatc ctgatcagac tgagcaacct
      601 gaccggcctg agcaacgact acagcagacg gaccatgggc ctgctggtgt ccgacatcgg
      661 caccatcgtg tggggcgcca caagcgccat ggccacaggc tacgtgaaag tgatcttctt
      721 ctgcctgggc ctgtgctacg gcgccaacac attcttccac gccgccaagg cctacatcga
      781 gggctaccac accgtgccca agggcagatg cagacaggtg gtcaccggca tggcctggct
      841 gttcttcgtg tcctggggca tgttccccat cctgttcatc ctgggccccg agggcttcgg
      901 cgtgctgtct gtgtacggca gcaccgtggg ccacaccatc atcgacctga tgagcaagaa
      961 ctgctgggga ctgctgggcc actacctgag agtgctgatc cacgagcaca tcctgatcca
     1021 cggcgatatc agaaagacca ccaagctgaa catcggcggc accgagatcg aggtggaaac
     1081 cctggtcgag gacgaggccg aggctggc
//
