LOCUS       sequence-121053-         842 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  His-GST-Smt3-NpuDnaEN102-CBD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 842)
  AUTHORS   Guerrero F, Ciragan A, Iwai H
  TITLE     Tandem SUMO fusion vectors for improving soluble protein expression 
            and purification.
  JOURNAL   Protein Expr Purif. 2015 Aug 20. pii: S1046-5928(15)30039-5. doi: 
            10.1016/j.pep.2015.08.019.
  PUBMED    26297996
REFERENCE   2  (bases 1 to 842)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif. 2015 Aug 20. pii: S1046-5928(15)30039-5. doi: 
            10.1016/j.pep.2015.08.019."
COMMENT     Sequence Label: pLJSRSF3
FEATURES             Location/Qualifiers
     source          1..842
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(15..59)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             complement(88..243)
                     /codon_start=1
                     /gene="Bacillus circulans chiA"
                     /product="chitin binding domain from chitinase A1 (Watanabe
                     et al., 1994)"
                     /label=CBD
                     /translation="TTNPGVSAWQVNTAYTAGQLVTYNGKTYKCLQPHTSLAGWEPSNV
                     PALWQLQ"
ORIGIN
        1 gctgcgctag tagacgagtc catgtgctgg cgttcaaatt tcgcagcagc ggtttcttta
       61 ccagactcga gtgcggccgc aagcttattg aagctgccac aaggcaggaa cgttggatgg
      121 ttcccatcct gccaaggagg tgtggggctg caaacattta tacgtcttgc cgttatatgt
      181 gaccaattgt cccgcagtat aagctgtgtt gacctgccaa gcggatacac caggatttgt
      241 ggtaccgaat tcgttcggca aattatcaac ccgcatcaaa tccaattcac gttcaaatat
      301 ttcatcaatt ggcaacattt gaccatcaac agtcataaac ttatggtctt ttgttgcccg
      361 aatcaatgaa ccatcttcca aacaatactc aaacacctct tgttctccgc gatcgtgcca
      421 ttgtgctaca ggttgtgtat aaatatttcc attattatca acgctataaa cagtacattc
      481 gatgcgcttt tctacaattt taccaatcgg taataatcca tattctactg tcaatatttc
      541 cgtttcatag cttaaacagg atccttccgt tacggtgtag gttttggtag cgtcgtcgta
      601 ggtccattca ccgtcaacac cgttgtcgtt agcgtactgt ttgaaaactt tttccgcggt
      661 agcagcgtca acagcttcgg tggtggtttc acctttcagg gttttaccgt tcaggataag
      721 tttgtactgc atatgggatc caccaatctg ttctctgtga gtctcaataa tatcgttatc
      781 ctccatgtcc aaatcttcag gggtctgatc agcttgaatt ctaataccgt cgtacaagaa
      841 tc
//
